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首页> 外文期刊>Journal of Nutritional Science and Vitaminology >PPARalpha and PPARdelta Transactivity and p300 Binding Activity Induced by Arachidonic Acid in Colorectal Cancer Cell Line Caco-2.
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PPARalpha and PPARdelta Transactivity and p300 Binding Activity Induced by Arachidonic Acid in Colorectal Cancer Cell Line Caco-2.

机译：花生四烯酸在大肠癌细胞系Caco-2中诱导的PPARalpha和PPARdelta转移性以及p300结合活性。

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摘要

It is reported that arachidonic acid strongly induces the conformational change in vitro and transactivity of PPARalpha in colorectal cancer cell line Caco-2. In this study, we demonstrated that the induction of conformational change and transactivity of PPARdelta by arachidonic acid, as well as other polyunsaturated fatty acids, was considerably lower than that of PPARalpha. Mammalian two-hybrid assay showed that arachidonic acid enhanced binding of one of the coactivators, p300, to PPARalpha but not to PPARdelta. Additionally, arachidonic acid induced in vitro binding of both PPARalpha-RXRalpha and PPARdelta-RXRalpha heterodimers to several PPREs on CRBPII, L-FABP and ACO genes. Our results suggest that the lower transactivity of PPARdelta for arachidonic acid in Caco-2 cells, compared with PPARalpha, is associated with the binding activity of p300 to the receptor.
机译：据报道，花生四烯酸在结直肠癌细胞系Caco-2中强烈诱导体外构象变化和PPARα的活性。在这项研究中，我们证明花生四烯酸以及其他多不饱和脂肪酸诱导的PPARδ构象变化和交易活性大大低于PPARalpha。哺乳动物两杂交试验表明，花生四烯酸增强了一种共激活因子p300与PPARalpha的结合，但与PPARdelta的结合没有。另外，花生四烯酸诱导PPARalpha-RXRalpha和PPARdelta-RXRalpha异二聚体与CRBPII，L-FABP和ACO基因上的几种PPRE的体外结合。我们的结果表明，与PPARalpha相比，Caco-2细胞中PPARdelta对花生四烯酸的较低交易活性与p300与受体的结合活性有关。

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