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首页> 外文期刊>Journal of Plant Pathology >A PHYTOPLASMA IS ASSOCIATED WITH THE WELIGAMA COCONUT LEAF WILT DISEASETI A PHYTOPLASMA IS ASSOCIATED WITH THE WELIGAMA COCONUT LEAF WILT DISEASE IN SRI LANKA
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A PHYTOPLASMA IS ASSOCIATED WITH THE WELIGAMA COCONUT LEAF WILT DISEASETI A PHYTOPLASMA IS ASSOCIATED WITH THE WELIGAMA COCONUT LEAF WILT DISEASE IN SRI LANKA

机译：斯威兰卡威椰子叶枯萎病与植物相关性斯威兰卡威椰子叶枯萎病与植物相关性

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摘要

Coconut palm (Cocos nucifera), is a major and economically important plantation crop widely cultivated in Sri Lanka. A sudden outbreak of a rapidly spreading non-lethal syndrome was first reported in coconut palms from southern Sri Lanka in 2006. This syndrome was named Weligama coconut leaf wilt disease (WCLWD) as it occurred in the Weligama area. WCLWD symptoms are flaccidity and marginal necrosis of leaflets and intense yellowing of the fronds, similar to the Kerala wilt disease of coconut in India. As the disease progresses the crown becomes smaller, the trunk begins to taper and the palm becomes unproductive. Abiotic factors and extra-cellular pathogens were discarded as the cause of WCLWD. Considering that a phytoplasma was previously associated with Kerala wilt disease, the possible phytoplasma aetiology of WCLWD was investigated. DNA extracted from midribs of spear leaves was subjected to nested PCR with phytoplasma universal primers R16F2n/R16R2 and R16mF2/R16R2 nested with fU5/rU3; P1/P7 nested with Chrfor/rU3; and direct PCR with Pc399/P1694. PCR products of expected sizes were obtained from diseased but not from healthy palms from a disease-free area. The sequences generated from the PCR products were submitted to similarity search (BlastN) in the NCBI database which confirmed that a phytoplasma belonging to the 16SrXI 'Candidatus Phytoplasma oryzae' group is associated with WCLWD. The phytoplasma was found to be highly similar but not identical to Sugarcane white leaf phytoplasma (99%), Sugarcane grassy shoot phytoplasma (99%) and Kerala wilt phytoplasma (99%).
机译：可可椰子（Cocos nucifera）是斯里兰卡广泛种植的一种重要且具有经济重要性的人工林。 2006年，斯里兰卡南部的椰子树首次爆发了快速传播的非致命综合症，该病突然爆发。这种综合症被称为Weligama椰子叶枯萎病（WCLWD），因为它发生在Weligama地区。 WCLWD症状是小叶松弛和边缘坏死以及叶状体严重发黄，类似于印度椰子的喀拉拉枯萎病。随着疾病的进展，冠变小，躯干开始逐渐变细，手掌变得无能为力。丢弃非生物因子和细胞外病原体作为WCLWD的原因。考虑到植物原虫以前与喀拉拉枯萎病有关，因此研究了WCLWD可能的植物原虫病学。从矛叶中脉中提取的DNA进行巢状PCR，使用植原体通用引物R16F2n / R16R2和R16mF2 / R16R2嵌套fU5 / rU3; P1 / P7与Chrfor / rU3嵌套;并使用Pc399 / P1694直接PCR。预期大小的PCR产物是从患病的地方获得的，而不是从无病地区的健康手掌中获得的。从PCR产物产生的序列被提交到NCBI数据库中的相似性搜索（BlastN），该数据库确认属于16SrXI'米氏假丝酵母（Candidatus Phytoplasma oryzae）组的植物原质与WCLWD相关。发现该植物质体与甘蔗白叶植物质体（99％），甘蔗草梢植物质体（99％）和喀拉拉枯萎植物质体（99％）高度相似但不相同。

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