首页> 外文期刊>Journal of Plant Physiology >COMPARISON OF SORBITOL TRANSPORT IN EXCISED TISSUE DISCS AND CORTEX TISSUE OF INTACT APPLE FRUIT
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COMPARISON OF SORBITOL TRANSPORT IN EXCISED TISSUE DISCS AND CORTEX TISSUE OF INTACT APPLE FRUIT

机译:山梨醇在正常组织圆盘中的运输与完整苹果皮层组织的比较

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摘要

Transport of [C-14]sorbitol from a bathing solution into isolated tissue discs of apple fruit was compared with transport of sorbitol supplied to wells cut in tissue of intact fruit. After uptake of sorbitol, labelled tissue was excised from fruit and, as for isolated discs, the tissue was washed in a hypotonic solution to remove label from the apoplast. This treatment also released radioactive label from the cytoplasm. The procedure allowed the separation of radioactivity located in the apparent free space, comprising apoplast and cytoplasm, from the radioactivity remaining in the compartmented space of the tissue.
机译:将[C-14]山梨糖醇从沐浴液中转移到苹果果实分离的组织圆盘中的过程与将山梨醇运送至完整水果组织中切下的孔中的转移过程进行了比较。吸收山梨糖醇后,从水果上切下标记的组织,就分离的椎间盘而言,在低渗溶液中洗涤组织以去除质外体中的标记。该处理还从细胞质中释放出放射性标记。该程序允许将位于表观自由空间(包括质外体和细胞质)中的放射性与保留在组织隔室中的放射性分离。

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