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首页> 外文期刊>Journal of liquid chromatography and related technologies >Simultaneous hplc analysis of palm carotenoids and tocopherols using a C-30 column and photodiode array detector
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Simultaneous hplc analysis of palm carotenoids and tocopherols using a C-30 column and photodiode array detector

机译:使用C-30色谱柱和光电二极管阵列检测器同时进行棕榈类胡萝卜素和生育酚的hplc分析

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摘要

An HPLC method for simultaneous identification and quantitationof tocopherols and carotenoids in red palm oil was developed. A C-30 column was used in a HPLC equipped with a photodiode detector and with methyl tertiary-butyl ether/methanol/water as the mobile phase. Tocopherols and carotenoids were monitored at 295 nm and 450 nm, respectively. Gradient clution resulted in complete separation of five tocopherols such as #alpha#, #delta#-, #gamma#-tocopherols and tocotrienols in palm oil in oless than 10 minutes. Several (13) carotenoids were also detected and quantitated in red palm oil within 45 minutes.
机译:建立了同时鉴别和定量红棕榈油中生育酚和类胡萝卜素的高效液相色谱方法。在装有光电二极管检测器并以甲基叔丁基醚/甲醇/水为流动相的HPLC中,使用C-30色谱柱。分别在295 nm和450 nm处监测生育酚和类胡萝卜素。梯度洗脱可在不到10分钟的时间内完全分离出棕榈油中的5种生育酚,例如#alpha#,#delta#-,#gamma#-生育酚和生育三烯酚。还可以在45分钟内在红棕榈油中检测到数种(13)类胡萝卜素并进行定量。

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