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首页> 外文期刊>Journal of liquid chromatography and related technologies >Measurement and pharmacokinetic study of gastrodin in rat blood by capillary micellar electrokinetic chromatography
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Measurement and pharmacokinetic study of gastrodin in rat blood by capillary micellar electrokinetic chromatography

机译：毛细管胶束电动色谱法测定大鼠血液中天麻素的含量及药代动力学研究

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摘要

A simple, accurate, and rapid capillary micellar electro-kinetic chromatography method was developed for the determination of gastrodin in rat blood. All experiments were performed in a 47cm (40cm effective length) x 75 < mu >m i.d. uncoated fused-silica capillary with UV detection at 200 nm. A running buffer composed of 50mM sodium tetraborate, 15 mM sodium dodecyl sulfate (SDS) (pH = 9.50) was found to be suitable. The method was linear in the range of 2.50 < similar-to > 200.0 < mu >g/mL (R = 0.999), and the detection limit was 0.50 < mu >g/mL. Only a small amount of blood (about 50 < mu >L) was required for each analysis. The recovery of gastrodin in rat blood was 91.51 < similar-to > 96.39%. This method was suitable for the determination of gastrodin after femoral vein injection and could be used for pharmaco-kinetic studies of this drug in rat blood.
机译：建立了一种简单，准确，快速的毛细管胶束电动色谱方法，用于测定大鼠血液中天麻素。所有实验均在47cm（有效长度40cm）×75μmm.d.下进行。未涂覆的熔融石英毛细管，可在200 nm处进行UV检测。发现由50mM四硼酸钠，15 mM十二烷基硫酸钠（SDS）（pH = 9.50）组成的运行缓冲液是合适的。该方法在2.50 <相似> 200.0 g / mL的范围内呈线性（R = 0.999），检出限为0.50 g / mL。每次分析仅需要少量血液（约50μL）。大鼠血液中天麻素的回收率为91.51 <相似-> 96.39％。该方法适用于股静脉注射后胃泌素的测定，可用于该药物在大鼠血液中的药代动力学研究。

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