• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Combined testing of antibody titer and affinity improves insulin autoantibody measurement: Diabetes Antibody Standardization Program.
【24h】

Combined testing of antibody titer and affinity improves insulin autoantibody measurement: Diabetes Antibody Standardization Program.

机译：抗体效价和亲和力的组合测试可改善胰岛素自身抗体的测量：糖尿病抗体标准化计划。

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of the workshop was to assess whether four laboratories could reproducibly measure insulin autoantibody (IAA) affinity in coded sera from non-diabetic relatives of patients with type 1 diabetes, newly diagnosed patients, and healthy blood donors, and whether combining affinity with autoantibody titer could improve concordance and performance of IAA assays. IAA affinity was measured by competitive binding using constant amounts of Tyr14A [125I] human insulin and increasing quantities of unlabeled human insulin. There was high concordance between laboratories in distinguishing high, moderate, and low affinity IAA, although IAA binding to insulin varied with assay format. Multiple islet autoantibody-positive and patient sera were identified by high affinity IAA regardless of laboratory-designated IAA status. Combining affinity and titer significantly improved sensitivity, specificity, and concordance of IAA measurement. This workshop has demonstrated that different laboratories are able to reproduce IAA affinity results and that considering IAA affinity is likely to improve the diagnostic performance of IAA assays.
机译：研讨会的目的是评估四个实验室是否可以可重现地测量1型糖尿病患者，新诊断患者和健康献血者的非糖尿病亲属在编码血清中的胰岛素自身抗体（IAA）亲和力，以及是否将亲和力与自身抗体相结合滴度可以提高IAA分析的一致性和性能。使用恒定量的Tyr14A [125I]人胰岛素和增加量的未标记人胰岛素，通过竞争性结合来测量IAA亲和力。实验室之间在区分高，中和低亲和力IAA方面存在高度一致性，尽管IAA与胰岛素的结合随测定形式而异。无论实验室指定的IAA状态如何，都可以通过高亲和力IAA鉴定出多个胰岛自身抗体阳性和患者血清。结合亲和力和效价可以显着提高IAA测量的灵敏度，特异性和一致性。该研讨会证明了不同的实验室能够再现IAA亲和力的结果，考虑IAA亲和力可能会改善IAA分析的诊断性能。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号