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首页> 外文期刊>Comparative Medicine >Transmission probabilities of mouse parvovirus 1 to sentinel mice chronically exposed to serial dilutions of contaminated bedding.
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Transmission probabilities of mouse parvovirus 1 to sentinel mice chronically exposed to serial dilutions of contaminated bedding.

机译：小鼠细小病毒1向哨兵小鼠的传播概率长期暴露于受污染的被褥的系列稀释液中。

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Intermittent serodetection of mouse parvovirus (MPV) infections in animal facilities occurs frequently when soiled bedding sentinel mouse monitoring systems are used. We evaluated induction of seroconversion in naive single-caged weanling ICR mice (n = 10 per group) maintained on 5-fold serially diluted contaminated bedding obtained from SCID mice persistently shedding MPV1e. Soiled bedding from the infected SCID mice was collected, diluted, and redistributed weekly to cages housing ICR mice to represent chronic exposure to MPV at varying prevalence in a research colony. Sera was collected every other week for 12 wk and evaluated for reactivity to MPV nonstructural and capsid antigens by multiplex fluorescent immunoassay. Mice were euthanized after seroconversion, and DNA extracted from lymph node and spleen was evaluated by quantitative PCR. Cumulative incidence of MPV infection for each of the 7 soiled bedding dilution groups (range, 1:5 to 1:78125 [v/v]) was 100%, 100%, 90%, 20%, 70%, 60%, and 20%, respectively. Most seropositive mice (78%) converted within the first 2 to 3 wk of soiled bedding exposure, correlating to viral exposure when mice were 4 to 7 wk of age. Viral DNA was detected in lymphoid tissues collected from all mice that were seropositive to VP2 capsid antigen, whereas viral DNA was not detected in lymphoid tissue of seronegative mice. These data indicate seroconversion occurs consistently in young mice exposed to high doses of virus equivalent to fecal MPV loads observed in acutely infected mice, whereas seroconversion is inconsistent in mice chronically exposed to lower doses of virus.
机译：当使用肮脏的床上用品前哨小鼠监测系统时，经常会在动物设施中间歇性检测小鼠细小病毒（MPV）感染。我们评估了幼稚的单笼断奶ICR小鼠（每组n = 10）中血清转化的诱导作用，该小鼠保持从连续脱落MPV1e的SCID小鼠获得的5倍连续稀释的受污染床上用品。每周收集，稀释和稀释来自感染SCID小鼠的被褥，并将其重新分配到容纳ICR小鼠的笼子中，以表示在研究菌落中以不同的患病率长期暴露于MPV。每隔一周收集一次血清，共12周，并通过多重荧光免疫测定法评估其对MPV非结构和衣壳抗原的反应性。血清转化后对小鼠实施安乐死，并通过定量PCR评估从淋巴结和脾中提取的DNA。 7个被污染的被褥稀释组（范围从1：5到1：78125 [v / v]）中MPV感染的累积发生率分别为100％，100％，90％，20％，70％，60％和分别为20％。大多数血清反应阳性的小鼠（78％）在被污染的床铺暴露的前2至3周内转化，与小鼠4至7周龄时的病毒暴露相关。在从对VP2衣壳抗原呈血清反应阳性的所有小鼠收集的淋巴组织中检测到病毒DNA，而在血清阴性小鼠的淋巴组织中未检测到病毒DNA。这些数据表明，血清转化在暴露于高剂量病毒的年轻小鼠中持续发生，这与急性感染小鼠中观察到的粪便MPV负荷相当，而血清转化在长期暴露于较低剂量病毒的小鼠中不一致。

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