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首页> 外文期刊>Comparative Medicine >Intestinal cytokine mRNA expression in canine inflammatory bowel disease: a meta-analysis with critical appraisal
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Intestinal cytokine mRNA expression in canine inflammatory bowel disease: a meta-analysis with critical appraisal

机译：犬炎症性肠病中肠细胞因子mRNA表达的Meta分析及评价

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Data implicating mucosal cytokines in the pathogenesis of canine inflammatory bowel disease (IBD) are limited. The aims of the present study were to report new findings of intestinal cytokine expression in dogs with IBD and to compare these data with previous studies through meta-analysis. Cytokine mRNA abundance in intestinal biopsies collected prospectively was evaluated by using a semiquantitative RT-PCR technique. For meta-analysis, an electronic database search revealed 3 clinical trials, all of which were nonrandomized (type III) case series. Prospective analysis showed that the intestines of healthy dogs and those with IBD express numerous cytokines and that a proinflammatory expression profile is not a feature of small or large-intestinal IBD. The meta-analysis data included 158 dogs characterized as healthy (n = 45), diarrheic nonIBD dogs (n = 6), nonresponders (n = 2), small-intestinal IBD (n = 41), colonic IBD (n = 25), and chronic enteropathy (n = 39). German shepherd dogs were overrepresented in 3 of the 4 studies. Healthy dogs showed mRNA expression for most cytokines including IL2, IL4, IL5, IL10, IL12, IFNgamma, TNFalpha, and TGFbeta. Only IL12 mRNA expression was increased consistently in small-intestinal IBD, whereas IBD colitis lacked consistent patterns of expression. In summary, dogs with IBD fail to express a predominant Th1- or Th2 cytokine bias in inflamed mucosa. Heterogeneity of results among these studies might be explained by numerous factors including the method of mRNA quantification, stage of disease, and demographic differences in study populations.
机译：在犬炎症性肠病（IBD）的发病机理中涉及粘膜细胞因子的数据是有限的。本研究的目的是报告IBD犬肠道细胞因子表达的新发现，并通过荟萃分析将这些数据与以前的研究进行比较。使用半定量RT-PCR技术评估前瞻性收集的肠活检组织中的细胞因子mRNA丰度。对于荟萃分析，电子数据库搜索显示了3项临床试验，所有这些试验均为非随机（III型）病例系列。前瞻性分析显示，健康犬和患有IBD的犬的肠表达大量细胞因子，而促炎性表达谱不是小肠或大肠IBD的特征。荟萃分析数据包括158条健康的狗（n = 45），腹泻的非IBD狗（n = 6），无反应者（n = 2），小肠IBD（n = 41），结肠IBD（n = 25）和慢性肠病（n = 39）。在四项研究中的三项中，德国牧羊犬的人数过多。健康的狗显示出大多数细胞因子的mRNA表达，包括IL2，IL4，IL5，IL10，IL12，IFNgamma，TNFalpha和TGFbeta。小肠IBD中仅IL12 mRNA表达持续增加，而IBD结肠炎缺乏一致的表达模式。总之，患有IBD的狗不能在发炎的粘膜中表达主要的Th1-或Th2细胞因子偏倚。这些研究结果的异质性可以通过许多因素来解释，包括mRNA定量方法，疾病阶段和研究人群的人口统计学差异。

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