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首页> 外文期刊>Comparative Medicine >Cytokine and Chemokine Responses of Lung Exposed to Surrogate Viral and Bacterial Infections
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Cytokine and Chemokine Responses of Lung Exposed to Surrogate Viral and Bacterial Infections

机译：替代病毒和细菌感染的肺的细胞因子和趋化因子反应

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The use of in vitro models of complex in vivo systems has yielded many insights into the molecular mechanisms that underlie normal and pathologic physiology However although the reduced complexity of these models is advantageous with regard to some research questions, the simplification may obscure or eliminate key influences that occur in vivo. We sought to examine this possibility with regard to the lung's response to infection, which may be inherent to resident lung cells or related to the systemic response to pulmonary infection. We used the inbred mouse strains C57BL/6J, DBA/2J, and B6.129S2-IL6(tm1Kopf), which differ in their response to inflammatory and infectious challenges, to assess in vivo responses of lung to surrogate viral and bacterial infection and compared these with responses of cultured lung slices and human A549 cells. Pulmonary cytokine concentrations were measured both after in vivo inoculation of mice and in vitro exposure of lung slices and A549 cells to surrogate viral and bacterial infections. The data indicate similarities and differences in early lung responses to in vivo compared with in vitro exposure to these inflammatory substances. Therefore, resident cells in the lung appear to respond to some challenges in a strain-independent manner, whereas some stimuli may elicit recruitment of peripheral inflammatory cells that generate the subsequent response in a genotype-related manner. These results add to the body of information pointing to host genotype as a crucial factor in mediating the severity of microbial infections and demonstrate that some of these effects may not be apparent in vitro.
机译：复杂体内系统的体外模型的使用已对构成正常生理和病理生理的分子机制产生了许多见解。尽管这些模型的复杂性降低了，但在某些研究问题上还是有利的，但简化可能会掩盖或消除关键影响在体内发生。我们试图从肺对感染的反应方面检查这种可能性，这可能是驻留肺细胞固有的，也可能与对肺部感染的全身反应有关。我们使用了自交系小鼠品系C57BL / 6J，DBA / 2J和B6.129S2-IL6（tm1Kopf），它们对炎症和感染性挑战的反应有所不同，以评估肺部替代病毒和细菌感染的体内反应并进行了比较这些与培养的肺切片和人类A549细胞的反应有关。在小鼠体内接种和肺切片和A549细胞体外暴露以替代病毒和细菌感染后，均测量了肺细胞因子浓度。数据表明与体外暴露于这些炎性物质相比，早期肺对体内反应的相似性和差异。因此，肺中的驻留细胞似乎以不依赖菌株的方式对某些挑战作出反应，而某些刺激可能引起周围炎症细胞的募集，这些炎症细胞以基因型相关的方式产生随后的反应。这些结果增加了指向宿主基因型的信息，这是介导微生物感染严重性的关键因素，并证明其中某些影响在体外可能并不明显。

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