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首页> 外文期刊>Analytica chimica acta >Multi-functional sample preparation procedure for measuring phytoestrogens in milk, cereals, and baby-food by liquid-chromatography tandem mass spectrometry with subsequent determination of their estrogenic activity using transcriptomic assay
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Multi-functional sample preparation procedure for measuring phytoestrogens in milk, cereals, and baby-food by liquid-chromatography tandem mass spectrometry with subsequent determination of their estrogenic activity using transcriptomic assay

机译：液相色谱串联质谱法测定牛奶，谷物和婴儿食品中植物雌激素的多功能样品制备程序，然后使用转录组分析方法测定其雌激素活性

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摘要

A method dedicated to the determination of a multiple range of phytoestrogens as endocrine disruptor compounds in infant food products was developed, with as double objective the specific measurement of 13 parameters and the evaluation of the estrogenic potency associated to this quantitative profile. A combined enzymatic and acidic chemical hydrolysis followed by a double purification on two successive C_(18) and SiOH Solid Phase Extraction cartridges permitted to efficiently purify milk, cereals and baby-food samples while eliminating naturally occurring estrogen hormones. A specific liquid chromatography-tandem mass spectrometric measurement authorised unambiguous identification and quantification of the target compounds. The proposed methodology was fully validated and applied to a set of around 30 real samples, demonstrating the presence of phytoestrogens at levels globally ranging from several μg kg~(-1) (ppb) to several tens mg kg~(-1) (ppm). The prepared sample extracts were proven to be suitable and compatible with the evaluation of their induced biological transcriptional activity on MCF-7 cell lines. Because permitting to cope with difficult issues such as low-dose and mixture effects, this proposed methodology may appear of particular interest for further exposure assessment studies and hazard characterisation investigations related to this class of endocrine disruptor compounds.
机译：开发了一种专门用于确定多种植物雌激素作为婴儿食品中内分泌干扰物的方法，其双重目标是对13个参数进行具体测量并评估与该定量谱相关的雌激素效能。酶和酸性化学水解相结合，然后在两个连续的C_（18）和SiOH固相萃取柱上进行两次纯化，可有效纯化牛奶，谷物和婴儿食品样品，同时消除天然存在的雌激素。特定的液相色谱-串联质谱测量技术可对目标化合物进行明确的鉴定和定量。所提出的方法已得到充分验证，并应用于一组约30个真实样品，表明存在的植物雌激素水平范围从几μgkg〜（-1）（ppb）到几十mg kg〜（-1）（ppm ）。所制备的样品提取物被证明是合适的，并且与它们在MCF-7细胞系上诱导的生物转录活性的评估兼容。因为可以处理低剂量和混合效应等难题，所以这种提议的方法对于与此类内分泌干扰物化合物有关的进一步暴露评估研究和危害特征研究可能显得特别有意义。

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