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首页> 外文期刊>Antimicrobial agents and chemotherapy. >Variability in telavancin cross-reactivity among vancomycin immunoassays
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Variability in telavancin cross-reactivity among vancomycin immunoassays

机译：万古霉素免疫测定法中特拉万星交叉反应的变异性

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Telavancin is a semisynthetic lipoglycopeptide with a dual mechanism of action against Gram-positive pathogens. Two brief reports have suggested potential cross-reactivity of telavancin with the vancomycin particle-enhanced turbidometric immunoassay (PETIA). The purpose of this study was to evaluate several commercially available vancomycin immunoassays (fluorescence polarization [FPIA], enzyme-multiplied immunoassays [EMIT], PETIA, and chemiluminescent immunoassay [CMIA]) for cross-reactivity with telavancin. Seven sites were selected to analyze serum samples for vancomycin. Each site received a set of samples (n = 18) which combined drug-free serum with telavancin, 7-OH telavancin metabolite, or vancomycin. Immunoassays demonstrating potential cross-reactivity were further evaluated by sending a duplicate sample set to multiple laboratories. Cross-reactivity was defined as the percent theoretical concentration (reported concentration/theoretical concentration × 100). No cross-reactivity was seen with FPIA or EMIT. Within the theoretical concentration range of 5 to 120 μg/ml of telavancin, the Synchron PETIA system reported vancomycin concentrations ranging from 4.7 to 54.2 μg/ml compared to vancomycin concentrations from 1.1 to 5.6 μg/ml for the Vista PETIA system. The Architect CMIA system reported vancomycin concentrations in the range of 0.27 to 0.97 μg/ml, whereas Advia Centaur XP CMIA reported vancomycin concentrations between 1.6 and 31.6 μg/ ml. The Architect CMIA immunoassay had the lowest percent cross-reactivity (0.8 to 5.4%), while the Synchron PETIA immunoassay demonstrated the highest percent cross-reactivity (45.2 to 53.8%). Telavancin samples measured by liquid chromatogra-phy- mass spectroscopy were within 93.9 to 122% of theoretical concentrations. Vancomycin concentrations were not measured in any 7-OH telavancin-spiked sample. Vancomycin concentrations measured by liquid chromatography-mass spectroscopy were within 57.2 to 113% of theoretical concentrations. PETIA and CMIA measured vancomycin concentrations in telavancinspiked samples. Significant variability in percent cross-reactivity was observed for each platform regardless of immunoassay method.
机译：特拉万星是一种半合成的脂肽，对革兰氏阳性病原体具有双重作用机制。两项简短的报告表明，特拉万星与万古霉素颗粒增强浊度免疫测定法（PETIA）可能发生交叉反应。这项研究的目的是评估几种可商购的万古霉素免疫测定法（荧光偏振[FPIA]，酶多重免疫测定法[EMIT]，PETIA和化学发光免疫测定法[CMIA]）与特拉万星的交叉反应性。选择七个部位来分析血清样品中的万古霉素。每个站点均接收一组样品（n = 18），这些样品将无药血清与替拉万星，7-OH替拉万星代谢物或万古霉素结合在一起。通过将重复的样品组发送到多个实验室，进一步评估了表明潜在交叉反应性的免疫分析方法。交叉反应性定义为理论浓度的百分比（报告浓度/理论浓度×100）。 FPIA或EMIT未见交叉反应。在理论上的特拉万星浓度为5至120μg/ ml的范围内，Synchron PETIA系统报告的万古霉素浓度为4.7至54.2μg/ ml，而VistaPETIA系统的万古霉素浓度为1.1至5.6μg/ ml。 Architect CMIA系统报告的万古霉素浓度为0.27至0.97μg/ ml，而Advia Centaur XP CMIA报告的万古霉素浓度为1.6至31.6μg/ ml。 Architect CMIA免疫测定的交叉反应百分比最低（0.8至5.4％），而Synchron PETIA免疫测定的交叉反应百分比最高（45.2至53.8％）。通过液相色谱-质谱法测定的特拉万星样品在理论浓度的93.9％至122％之内。在任何掺有7-OH特拉万星的样品中均未检测到万古霉素浓度。通过液相色谱-质谱法测定的万古霉素浓度在理论浓度的57.2％至113％之间。 PETIA和CMIA测量了掺入telavancin的样品中万古霉素的浓度。不管采用何种免疫测定方法，每个平台的交叉反应百分率均存在显着差异。

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