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首页> 外文期刊>Vaccine >First assessment of classical swine fever marker vaccine candidate CP7_E2alf for oral immunization of wild boar under field conditions.
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First assessment of classical swine fever marker vaccine candidate CP7_E2alf for oral immunization of wild boar under field conditions.

机译：对野外条件下野猪口服免疫的经典猪瘟标记疫苗候选CP7_E2alf的首次评估。

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Oral vaccination against classical swine fever (CSF) is a potent tool to control disease outbreaks in wild boar. So far, vaccination campaigns have been carried out using live attenuated vaccines that do not allow serological differentiation of infected from vaccinated animals (DIVA). Although this drawback is acceptable for wild boar, the use of marker vaccines would facilitate studies on disease and vaccination dynamics. Recently, the CSF marker vaccine candidate CP7_E2alf was assessed for oral immunization under laboratory conditions. Promising results prompted efforts to study the vaccine candidate under field conditions and in bait formulation. In this context, two oral vaccination campaigns were carried out with CP7_E2alf bait vaccines in two areas called 'faunistic-hunting farms' in the region of Umbria, Italy. One campaign was conducted using single vaccination, the second with the routinely employed double vaccination strategy. Both campaigns were carried out before concerted hunting actions were performed. Bait uptake, vaccine virus detection and antibody responses were assessed along with inspections upon gutting. As a comparator, seven wild boar were hand-fed with baits under laboratory conditions. In the field, bait uptake ranged from 63.7% to 98.7%, whereas antibody prevalence reached only 33.3-35.1%. The marker serology showed a strong influence of sample quality on the test outcome with a total of 85% of samples being classified correctly. Vaccine virus was not detectable. Under hand feeding conditions, six out of seven wild boar took up at least one bait, and five of them showed detectable antibody levels seven weeks after vaccination. These results were supplemented by stability tests. Appropriate stability of vaccine virus was shown both under field and laboratory conditions. In total, most results were in line with our expectations. However, optimization of the DIVA assay has to be attempted in the future.
机译：口服经典猪瘟疫苗是控制野猪疾病暴发的有效工具。迄今为止，已经使用减毒活疫苗进行了疫苗接种运动，该减毒活疫苗不允许从疫苗接种的动物（DIVA）进行血清学区分。尽管此缺点对于野猪是可以接受的，但使用标记疫苗将有助于疾病和疫苗接种动态的研究。最近，评估了CSF标志疫苗候选CP7_E2alf在实验室条件下的口服免疫效果。有希望的结果促使人们努力在野外条件和诱饵配方中研究候选疫苗。在这种情况下，在意大利翁布里亚地区的两个称为“动物狩猎场”的地区，开展了两次使用CP7_E2alf诱饵疫苗的口服疫苗运动。一个运动是使用单次疫苗接种进行的，第二个运动是使用常规采用的双重疫苗接种策略进行的。两次运动都是在进行一致的狩猎行动之前进行的。评估肠道吸收时的诱饵吸收，疫苗病毒检测和抗体反应。作为比较，在实验室条件下用饵料人工喂养了7只野猪。在田间，诱饵的摄取范围为63.7％至98.7％，而抗体的流行率仅为33.3-35.1％。标记血清学显示出样品质量对测试结果的强烈影响，总共有85％的样品被正确分类。无法检测到疫苗病毒。在人工喂养条件下，七只野猪中有六只至少捕获了一个诱饵，其中五只在接种疫苗七周后显示出可检测的抗体水平。这些结果通过稳定性测试得到补充。在野外和实验室条件下均显示出适当的疫苗病毒稳定性。总体而言，大多数结果都符合我们的预期。但是，将来必须尝试优化DIVA分析。

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