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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Analysis of Phospholipids in Infant Formulas Using High Performance Liquid Chromatography-Tandem Mass Spectrometry
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Analysis of Phospholipids in Infant Formulas Using High Performance Liquid Chromatography-Tandem Mass Spectrometry

机译：高效液相色谱-串联质谱法分析婴儿配方食品中的磷脂

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摘要

Phospholipids have been used widely in the food industry as emulsifiers, but it is their biological and nutritional function that has been the focus over recent years. The recognition of the importance of phospholipids and sphingomyelin for infant development has led to an increase in the number of infant formulas claiming to contain these complex lipid components. Therefore, the ability to measure these lipids in infant formulas and dairy-derived complex lipid ingredients for fortification purposes is important. A high-performance liquid chromatography—tandem mass spectrometry method that quantifies phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, and sphingomyelin found in infant formulas and dairy-derived complex lipid ingredients is described. The method uses external standards of similar fatty acid profile for calibration. The recovery of phospholipids ranged from 92% to 102% with a method repeatability coefficient of variation of 6—10%. In addition to the specificity and selectivity of the method, details of the molecular species in the individual phospholipid classes are available using this method.
机译：磷脂已在食品工业中广泛用作乳化剂，但近年来它们的生物学和营养功能一直是人们关注的焦点。人们认识到磷脂和鞘磷脂对婴儿发育的重要性，导致声称含有这些复杂脂质成分的婴儿配方食品数量增加。因此，为了强化目的而测定婴儿配方食品和乳制品衍生的复合脂质成分中这些脂质的能力很重要。描述了一种高效液相色谱-串联质谱法，该方法定量了婴儿配方食品和乳制品衍生的复合脂质成分中发现的磷脂酰胆碱，磷脂酰乙醇胺，磷脂酰肌醇，磷脂酰丝氨酸和鞘磷脂。该方法使用具有相似脂肪酸谱的外标进行校准。磷脂的回收率为92％至102％，方法的重复性变异系数为6-10％。除了该方法的特异性和选择性外，使用该方法还可以获得单个磷脂类别中分子种类的详细信息。

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