• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nanotechnology >Ampicillin-mediated functionalized gold nanoparticles against ampicillin-resistant bacteria: strategy, preparation and interaction studies
【24h】

Ampicillin-mediated functionalized gold nanoparticles against ampicillin-resistant bacteria: strategy, preparation and interaction studies

机译：氨苄青霉素介导的官能化金纳米颗粒对氨苄青霉素抗性细菌：策略，制备和相互作用研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Antibiotic resistance is a highly challenging concern of infectious diseases, and it requires a rational approach to overcome. Through this work, we have synthesized ampicillin-capped gold nanoparticles (Amp-Au NPs) and studied its interaction with bacterial cells. In this process of synthesis, the primary amine group of ampicillin acts as both reducing as well as capping agent. In addition to synthesized gold nanoparticles, the beta-lactam ring remains free to interact with bacteria. This approach not only utilizes the maximum efficiency of nanoparticles and antibiotics towards ampicillin sensitive bacterial cells but also proves to be effective against ampicillin resistance bacteria. Our results illustrate that the optimized system of Amp-Au NPs was formulated by taking 1.25 mM ampicillin and 10(-2) of gold ions concentration. UV-vis spectrum of gold nanoparticles and the presence of ampicillin were recorded at around 540 nm and 259 nm, respectively. Microscopic images indicate that particles are nearly spherical and are in size range between 25 and 50 nm. Moreover, formulated Amp-Au NPs show successful accumulation onto the surface of the bacterial cell as a result of which pores were formed into the bacterial membrane. The entry of nanoparticles into bacterial cells was validated through both atomic force microscopy and fluorescent microscopy. The adhesive properties of this coating material and its stability in various pH, i.e. pH 3, pH 7 and pH 10 conditions, could make them a good candidate in the prevention of biofilm formation. Amp-Au NPs show promising antimicrobial activity against ampicillin resistance Escherichia coli bacteria. Furthermore, antimicrobial studies indicate that the efficacy of Amp-Au NPs increased against both ampicillin sensitive and ampicillin resistance bacteria up to sixteen folds and four folds respectively.
机译：抗生素耐药性是传染病的一个极具挑战性的关注，它需要克服的理性态度。通过这项工作，我们合成氨苄青霉素皑皑的金纳米粒子（安培 - 金纳米粒子），并研究了细菌细胞的相互作用。在合成的过程中，氨苄西林的伯胺基团用作既降低以及封端剂。除了合成的金纳米颗粒，所述β-内酰胺环保持自由与细菌相互作用。这种方法不仅利用纳米颗粒和抗生素的朝向氨苄青霉素敏感的细菌细胞的最大效率，而且也被证明是有效对抗氨苄青霉素抗性菌。我们的结果表明，安培 - 金纳米粒子的优化的系统是由金离子浓度的服用1.25mM的氨苄青霉素和10（-2）配制。的UV-vis金纳米颗粒和氨苄青霉素的存在下的光谱分别在540nm附近和259纳米，记录。显微图像表明，颗粒是接近球形的，并且在25和50nm之间的尺寸范围。而且，配制的安培 - 金纳米粒子显示成功积累到作为结果的细菌细胞，其的孔形成到细菌膜的表面上。纳米颗粒引入细菌细胞的条目都是穿过原子力显微镜和荧光显微镜验证。该涂层材料及其在各种pH稳定性，即pH为3，pH为7和pH 10的条件下，所述的粘合特性可能使他们的良好候选预防生物膜形成。安培金纳米粒子显示有为对氨苄青霉素抗性的大肠杆菌的抗菌活性。此外，抗菌研究表明，安培 - 金纳米粒子的功效氨苄西林敏感和氨苄西林耐药细菌分别增加对阵双方多达十六倍和四个褶皱。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号