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首页> 外文期刊>RSC Advances >Enteric pH responsive cargo release from PDA and PEG coated mesoporous silica nanoparticles: a comparative study in Drosophila melanogaster
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Enteric pH responsive cargo release from PDA and PEG coated mesoporous silica nanoparticles: a comparative study in Drosophila melanogaster

机译：从PDA和PEG涂层中孔二氧化硅纳米粒子的肠溶pH响应性货物释放：果蝇Melanogaster的比较研究

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Physiological stimulus-specific cargo release from nanoparticle carriers is a holy grail of drug delivery research. While the majority of such work is carried out in vitro with cell lines, widespread use of common mammalian model systems - mice and rats - is difficult due to the associated cost and regulatory restrictions. Here we use the inexpensive, easily reared, excellent genetic model system Drosophila melanogaster to test pH responsive cargo release from widely used mesoporous silica nanoparticles (MSNs) coated with pH sensitive polydopamine (PDA) and polyethylene glycol (PEG) polymers. We synthesized 650 +/- 75 nm diameter PDA or PEG coated mesoporous silica nanoparticles loaded with a fluorescent dye and fed to individual adult flies. Subsequently, the passage of the particles were monitored through the fly gut. As in mammals, the fly intestine has multiple pH specific zones that are easily accessible for imaging and also genetic, biochemical or physiological manipulations. We observed that both the species of MSNs ruptured around the acidic (pH < 4.0) middle midgut of the flies. PEG coated particles showed sharper specificity of release in the acidic middle midgut of flies than the PDA coated ones and had less tendency to clump together. Our results clearly show that the Drosophila gut can be used as a model to test pH responsive biocompatible materials in vivo. Our work paves the way for greater use of Drosophila as an in vivo complete systemic model in drug delivery and smart materials research. It also suggests that such specific delivery of chemical/biological cargo can be exploited to study basic biology of the gut cells and their communication with other organs.
机译：纳米粒子载体的生理刺激特异性货物释放是药物递送研究的圣杯。虽然大多数此类作品在体外进行细胞系进行，但由于相关的成本和监管限制，广泛使用常见的哺乳动物模型系统 - 小鼠和大鼠 - 是困难的。在这里，我们使用廉价，易于饲养的优异的遗传模型系统果蝇从涂有pH敏感的聚德莫（PDA）和聚乙二醇（PEG）聚合物的广泛使用的介孔二氧化硅纳米粒子（MSN）中测试pH响应性货物释放。我们合成650 +/- 75nm直径的PDA或PEG涂覆的中孔二氧化硅纳米粒子，其装有荧光染料并加入单个成年苍蝇。随后，通过翼肠监测颗粒的通过。与哺乳动物一样，飞肠肠有多种pH特定区域，可容易地进行成像，也可以遗传，生化或生理操作。我们观察到，MSNS的种类周围的酸性（pH <4.​​0）中间中间菌落中的中间菌落破裂。 PEG涂覆的颗粒在恒定的酸性中间菌中释放的释放表现得比PDA涂覆的颗粒更高，并且倾向于丛集的倾向。我们的结果清楚地表明，果蝇肠可以用作测试体内pH响应性生物相容性材料的模型。我们的工作铺平了德罗芯的方式，以便在药物交付和智能材料研究中为体内完整的全身模型。它还表明，可以利用这种特定的化学/生物货物递送来研究肠细胞的基本生物学及其与其他器官的沟通。

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  • 来源
    《RSC Advances》 |2020年第20期|共11页
  • 作者

    Sapre Nidhi; Chakraborty Rusha; Purohit Poorvi; Bhat Suresh; Das Gaurav; Bajpe Sneha R.;

  • 作者单位

    SIU Symbiosis Ctr Nanosci &

    Nanotechnol Pune Maharashtra India;

    Natl Ctr Cell Sci Pune Maharashtra India;

    CSIR Natl Chem Lab Pune Maharashtra India;

    CSIR Natl Chem Lab Pune Maharashtra India;

    Natl Ctr Cell Sci Pune Maharashtra India;

    SIU Symbiosis Ctr Nanosci &

    Nanotechnol Pune Maharashtra India;

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