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首页> 外文期刊>RSC Advances >Panax notoginseng saponins radiosensitize colorectal cancer cells by regulating the SNHG6/miR-137 axis
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Panax notoginseng saponins radiosensitize colorectal cancer cells by regulating the SNHG6/miR-137 axis

机译：Panax Noginseng Saponins通过调节SnHG6 / miR-137轴来放射性敏感结肠直肠癌细胞

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Panax notoginseng saponins (PNS) have recently attracted great attention for their anti-cancer activity in colorectal cancer (CRC). The aim of this study was to explore the functional role and underlying mechanisms of PNS on CRC radiosensitivity. Cell viability was assessed by a Cell Counting kit-8 assay. Cell survival and apoptosis were determined using colony formation assay and flow cytometry, respectively. Quantitative real-time PCR was used to quantify the levels of SNHG6 and miR-137. The targeted correlation between SNHG6 and miR-137 was validated by dual-luciferase reporter and RNA immunoprecipitation assays. Our data supported that PNS weakened the viability of CRC cells. Moreover, PNS promoted the radiosensitivity of CRC cells. Mechanistically, PNS enhanced CRC cell radiosensitivity by upregulating SNHG6. SNHG6 directly targeted miR-137 and inhibited miR-137 expression. MiR-137 was involved in the regulatory effect of SNHG6 on CRC cell radiosensitivity. Furthermore, PNS increased miR-137 expression through SNHG6 in CRC cells. Our study suggested that PNS promoted radiosensitivity in CRC cells at least partly through regulating the SNHG6/miR-137 axis, providing a novel understanding of the anti-cancer mechanism of PNS in CRC.
机译：Panax Noginseng Saponins（PNS）最近引起了对整中性癌症（CRC）的抗癌活性的重视。本研究的目的是探讨PNS对CRC放射敏感性的功能作用和潜在机制。通过计数试剂盒-8测定评估细胞活力。使用菌落形成测定和流式细胞术测定细胞存活和细胞凋亡。定量实时PCR用于量化SnHG6和miR-137的水平。通过双荧光素酶报告和RNA免疫沉淀测定验证SNHG6和MIR-137之间的靶向相关性。我们的数据支持PNS削弱了CRC细胞的可行性。此外，PNS促进了CRC细胞的放射敏感性。机械地，PNS通过上调SNHG6来增强CRC细胞放射敏感性。 SNHG6直接针对miR-137并抑制miR-137表达。 MIR-137参与了SNHG6对CRC细胞放射敏感性的调节作用。此外，PNS通过CRC细胞中的SNHG6增加miR-137表达。我们的研究表明，PNS至少部分地通过调节SNHG6 / miR-137轴来促进CRC细胞的放射敏感性，为CRC中对PNS的抗癌机制提供了新的理解。

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    《RSC Advances》 |2019年第66期|共10页
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  • 正文语种 eng
  • 中图分类 化学;
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