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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Lipophilization of Hydroxytyrosol-Enriched Fractions from Olea europaea L. Byproducts and Evaluation of the in Vitro Effects on a Model of Colorectal Cancer Cells
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Lipophilization of Hydroxytyrosol-Enriched Fractions from Olea europaea L. Byproducts and Evaluation of the in Vitro Effects on a Model of Colorectal Cancer Cells

机译:羟基醇富含羟基铕的脂肪化从 opereoeaa-/ i> L.副产物和对成分癌细胞模型的体外影响的评价

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src="http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2017/jafcau.2017.65.issue-31/acs.jafc.6b05457/20170803/images/medium/jf-2016-05457p_0007.gif">A hydroxytyrosol (HTyr)-enriched fraction containing HTyr 6% w/w, derived from Olea europaea L. byproducts and obtained using an environmentally and economically sustainable technology, was lipophilized under green chemistry conditions. The effects of three fractions containing hydroxytyrosyl butanoate, octanoate, and oleate, named, respectively, lipophilic fractions 5, 6, and 7, and unreacted HTyr on the human colon cancer cell line HCT8-β8 engineered to overexpress estrogen receptor β (ERβ) were evaluated and compared to those of pure HTyr. The experimental data demonstrated that HTyr and all fractions showed an antiproliferative effect, as had been observed by the evaluation of the cellular doubling time under these different conditions (mean control, 32 ± 4 h; HTyr 1, 65 ± 9 h; fraction 5, 64 ± 11 h; fraction 6, 62 ± 14 h; fraction 7, 133 ± 30 h). As evidenced, fraction 7 containing hydroxytyrosyl oleate showed the highest activity. These results were related to the link with ER-β, which was assessed through simultaneous treatment with an inhibitor of ERβ.
机译:src =“http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2017/jafcau.2017.65.issue-31/acs.jafc.6b05457/20170803/20170803/20170803/20170803/images/medium / jf-2016-05457p_0007.gif“>羟基葡萄醇(HTYR) - 含有HTYR 6%w / w的甲羟基醇(HTYR)含有HTYR 6%w / w的馏分,衍生自欧洲欧洲欧洲欧洲欧洲葡萄糖副产物,并使用环境和经济上可持续的技术获得,在绿色化学条件下脂质化。含有羟基羟基甲酯,辛酸和油酸酯的三个级分的效果,分别,亲脂性级分5,6和7,并在人结肠癌细胞系HCT8-β8上进行了未反应的HTYR,工程化为过表达雌激素受体β(ERβ)是评估并与纯HTYR的评估。实验数据表明,HTYR和所有级分表现出抗增殖效应,如在这些不同条件下的细胞倍增时间的评价(平均控制,32±4小时; HTYR 1,65±9 H;级分5, 64±11小时;级分6,62±14小时;级分7,133±30 h)。如证明,含有羟基吡喃烯醇的级分7显示出最高的活性。这些结果与与ER-β的联系有关,通过用ERβ的抑制剂同时治疗来评估。

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