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首页> 外文期刊>Journal of Molecular Biology >The Selenium Transport Protein, Selenoprotein P, Requires Coding Sequence Determinants to Promote Efficient Selenocysteine Incorporation
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The Selenium Transport Protein, Selenoprotein P, Requires Coding Sequence Determinants to Promote Efficient Selenocysteine Incorporation

机译：硒转运蛋白酶硒蛋白P.需要编码序列决定簇，以促进有效的硒细胞含量

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摘要

Selenoproteins are an essential and unique group of proteins in which selenocysteine (Sec) is incorporated in response to a stop codon (UGA). Reprograming of UGA for Sec insertion in eukaryotes requires a cis-acting stem loop structure in the 3' untranslated region of selenoprotein mRNA and several trans-acting factors. Together these factors are sufficient for Sec incorporation in vitro, but the process is highly inefficient. An additional challenge is the synthesis of selenoprotein P (SELENOP), which uniquely contains multiple UGA codons. Full-length SELENOP expression requires processive Sec incorporation, the mechanism for which is not understood. In this study, we identify core coding region sequence determinants within the SELENOP mRNA that govern SELENOP synthesis. Using Se-75 labeling in cells, we determined that the N-terminal coding sequence (upstream of the second UGA) and C-terminal coding sequence context are two independent determinants for efficient synthesis of full-length SELENOP. In addition, the distance between the first UGA and the consensus signal peptide is also critical for efficiency. (C) 2018 Elsevier Ltd. All rights reserved.
机译：硒蛋白是一种必要而独特的蛋白质组，其中硒细胞酮（SEC）响应于止芯密码子（UGA）。用于在真核生物中插入的UGA的重新编程需要在硒蛋白mRNA的3'未翻译区域中的顺式作用杆环结构和几种反式作用因子。这些因素在体外，这些因素足以掺入，但该过程是高效的。额外的挑战是Selenoprotein P（Selenop）的合成，其唯一地含有多个UGA密码子。全长SELENOP表达需要加工部队委员会的合并，该机制尚未理解。在该研究中，我们鉴定了治治Selenop合成的Selenop mRNA内的核心编码区序列序列。在细胞中使用SE-75标记，确定N-末端编码序列（第二UGA的上游）和C末端编码序列上下文是两个独立的决定簇，用于高效合成全长SELENOP。此外，第一UGA与共有信号肽之间的距离也是效率的关键。 （c）2018年elestvier有限公司保留所有权利。

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