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首页> 外文期刊>Biosciences Biotechnology Research Asia >Phytochemical Composition and In vitro Antimicrobial, Antioxidant Activites of Methanolic Leaf Extracts From Excoecaria agallocha
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Phytochemical Composition and In vitro Antimicrobial, Antioxidant Activites of Methanolic Leaf Extracts From Excoecaria agallocha

机译：墨西哥Excoecaria agallocha甲醇叶提取物的植物化学组成和体外抗微生物，抗氧化活性

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摘要

This study investigated the phytochemical, antimicrobial and antioxidant properties of methanolic leaf fractionated extracts of Excoecaria agallocha. Antibacterial activity test of fractionated extract of Excoecaria agallocha was conducted via Disk diffusion assay. Micro titer broth dilution technique was performed to determine the minimum inhibitory concentration (MIC) and Minimum Bactericidal Concentration (MBC). Qualitative and quantitative DPPH radical scavenging activity determines the antioxidant activity in E. agallocha. Chromatographic separation was completed using thin layer chromatography (TLC) and column chromatographic techniques. Phytochemical screening of fractionated extract revealed active constituents including alkaloids, saponins,flavonoids, terpenoid and tannins. In vitro antibacterial activity of column-fractionated extract demonstrated inhibitory effect against Flavobacterium indicum, Chryseobacterium meningosepticum, Chryseobacterium indologenes and Chryseobacterium gleam isolated from diseased fish. MIC and MBC of E. agallocha fractionated extract was 1.56 mg/ml and 3.12 mg/ml, respectively. TLC-based qualitative DPPH analysis revealed antioxidant activity in E. agallocha extract, specified by yellowish stain on maroon color TLC plate. These bands indicate the presence of various compounds that could act as potential antimicrobial agents. The present study indicates that E. agallocha leaves contain active phytochemicals which inhibit bacteria and could be useful in the treatment of microbial infections in fish.
机译：这项研究调查了Excoecaria agallocha的甲醇叶片分级提取物的植物化学，抗微生物和抗氧化特性。通过圆盘扩散法进行了Exocecaria agallocha分级提取物的抗菌活性测试。进行微量滴定液稀释技术以确定最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。定性和定量的DPPH自由基清除活性决定了紫花大戟中的抗氧化活性。使用薄层色谱（TLC）和柱色谱技术完成色谱分离。分级提取物的植物化学筛选显示出活性成分，包括生物碱，皂角苷，类黄酮，萜类和丹宁酸。柱分离的提取物的体外抗菌活性证明对从病鱼中分离出的印度黄杆菌，脑膜炎杆菌，产吲哚酸杆菌和微光杆菌有抑制作用。紫花苜蓿分级提取物的MIC和MBC分别为1.56 mg / ml和3.12 mg / ml。基于TLC的定性DPPH分析表明，紫红色的E.agallocha提取物中的抗氧化活性由褐红色TLC板上的淡黄色斑点表示。这些谱带表明存在各种可能用作潜在抗菌剂的化合物。目前的研究表明，紫花苜蓿叶中含有抑制细菌的活性植物化学物质，可用于治疗鱼类中的微生物感染。

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