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首页> 外文期刊>Acta biomaterialia >Fabrication of a model continuously graded co-electrospun mesh for regeneration of the ligament-bone interface.
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Fabrication of a model continuously graded co-electrospun mesh for regeneration of the ligament-bone interface.

机译：模型的连续渐变共电纺网的制造，用于韧带-骨界面的再生。

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Current scaffolds for the regeneration of anterior cruciate ligament injuries are unable to capture intricate mechanical and chemical gradients present in the natural ligament-bone interface. As a result, stress concentrations can develop at the scaffold-bone interface, leading to poor osseointegration. Hence, scaffolds that possess appropriate mechano-chemical gradients would help establish normal loading properties at the interface, while promoting scaffold integration with bone. With the long-term goal of investigating regeneration of the ligament-bone interface, this feasibility study aimed to fabricate a continuously graded mesh. Specifically, graded meshes were fabricated by co-electrospinning nanohydroxyapatite/polycaprolactone (nHAP-PCL) and poly(ester urethane) urea elastomer solutions from offset spinnerets. Next, mineral crystallites were selectively deposited on the nHAP-PCL fibers by treatment with a 5x simulated body fluid (5x SBF). X-ray diffraction and energy-dispersive spectroscopy indicated calcium-deficient hydroxyapatite-like mineral crystallites with an average Ca/P ratio of 1.48. Tensile testing demonstrated the presence of a mechanical gradient, which became more pronounced upon treatment with 5x SBF. Finally, biocompatibility of the graded meshes was verified using an MC3T3-E1 osteoprogenitor cell line. The study demonstrates that graded meshes, for potential application in interfacial tissue engineering, can be fabricated by co-electrospinning.
机译：当前用于再生前交叉韧带损伤的支架不能捕获天然韧带-骨界面中存在的复杂的机械和化学梯度。结果，应力集中会在支架-骨界面处发展，导致骨整合不良。因此，具有适当的机械化学梯度的支架将有助于在界面处建立正常的加载特性，同时促进支架与骨骼的整合。出于研究韧带-骨界面再生的长期目标，此可行性研究旨在制造连续渐变的网格。具体来说，通过将静电纺丝的纳米羟基磷灰石/聚己内酯（nHAP-PCL）和聚（酯氨基甲酸酯）尿素弹性体溶液进行共电纺丝，可以制造出渐变网格。接下来，通过用5倍模拟体液（5倍SBF）处理，将矿物微晶选择性沉积在nHAP-PCL纤维上。 X射线衍射和能量色散光谱显示钙缺乏的羟基磷灰石样矿物微晶，平均Ca / P比为1.48。拉伸测试表明存在机械梯度，在用5倍SBF处理后，机械梯度变得更加明显。最后，使用MC3T3-E1骨祖细胞系验证了渐变网格的生物相容性。这项研究表明，可以通过共电纺丝制造在界面组织工程中潜在应用的渐变网格。

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