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首页> 外文期刊>Acta biomaterialia >Morphological analysis of the antimicrobial action of nitric oxide on gram-negative pathogens using atomic force microscopy.
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Morphological analysis of the antimicrobial action of nitric oxide on gram-negative pathogens using atomic force microscopy.

机译：使用原子力显微镜对一氧化氮对革兰氏阴性病原体的抗菌作用进行形态学分析。

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摘要

Atomic force microscopy (AFM) was used to study the morphological changes of two gram-negative pathogens, Pseudomonas aeruginosa and Escherichia coli, after exposure to nitric oxide (NO). The time-dependent effects of NO released from a xerogel coating and the concentration-dependent effects rendered by a small molecule that releases NO in a bolus were examined and compared. Bacteria exhibited irregular and degraded exteriors. With NO-releasing surfaces, an increase in surface debris and disorganized adhesion patterns were observed compared to controls. Analysis of cell surface topography revealed that increasing membrane roughness correlated with higher doses of NO. At a lower total dose, NO delivered via a bolus resulted in greater membrane roughness than NO released from a surface via a sustained flux. At sub-inhibitory levels, treatment with amoxicillin, an antibiotic known to compromise the integrity of the cell wall, led to morphologies resembling those resulting from NO treatment. Our observations indicate that cell envelope deterioration is a visible consequence of NO-exposure for both gram-negative species studied.
机译：原子力显微镜（AFM）用于研究两种革兰氏阴性病原体铜绿假单胞菌和大肠杆菌在暴露于一氧化氮（NO）后的形态变化。检查并比较了由干凝胶涂层释放的NO的时间依赖性效应和以大剂量释放NO的小分子产生的浓度依赖性效应。细菌表现出不规则和退化的外观。与对照相比，在具有释放NO的表面的情况下，观察到表面碎屑的增加和混乱的粘附模式。细胞表面形貌的分析表明，增加的膜粗糙度与较高剂量的NO有关。在较低的总剂量下，与通过持续通量从表面释放的NO相比，通过大剂量释放的NO导致更大的膜粗糙度。在亚抑制水平下，用阿莫西林（一种已知会损害细胞壁完整性的抗生素）进行治疗，会导致产生类似于NO治疗所导致的形态。我们的观察结果表明，对于两个革兰氏阴性菌种，细胞包膜的降解都是NO暴露的明显结果。

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