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首页> 外文期刊>RSC Advances >The use of isocyanide-based multicomponent reaction for covalent immobilization of Rhizomucor miehei lipase on multiwall carbon nanotubes and graphene nanosheets
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The use of isocyanide-based multicomponent reaction for covalent immobilization of Rhizomucor miehei lipase on multiwall carbon nanotubes and graphene nanosheets

机译：基于异氰酸盐的多组分反应对多壁碳纳米管和石墨烯纳米液中的根瘤菌的共价固定反应

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摘要

We describe here a novel and simple method for making bioconjugation and immobilization of Rhizomucor miehei lipase (RML) on carboxylated multiwall carbon nanotubes (MWCNT-COOH) and carboxylated graphene nanosheets (Gr-COOH) by using an isocyanide-based four-component reaction. In this approach, the enzyme supplies amino groups, the support supplies carboxylic acid groups, and the missing components (isocyanide and aldehyde) are added to the reaction medium. This coupling reaction was carried out in water at 25 degrees C, in which rapid and high enzyme loading were achieved. The maximum loading capacity of 530 mg and 680 mg was obtained for Gr-COOH and MWCNT-COOH, respectively. A variety of techniques including FTIR, Raman spectroscopy, XRD, SEM, and TGA were employed to characterize the immobilized derivatives of RML. The immobilized preparations showed significantly increased thermal stability and co-solvent stability as compared to the soluble enzyme. Kinetic parameters and optimum pH activity of RML and its immobilized preparations were also determined. The K-m values of 0.44, 0.23, and 0.18 mM and the maximum reaction rates (V-max) of 0.09, 0.1, and 0.08 mM min(-1) were obtained for MWCNTs-RML, Gr-RML, and free RML, respectively. This approach may provide a general and efficient method to attach biomolecules on a variety of carboxylated solid surfaces at ambient conditions.
机译：在这里，我们描述了一种新颖和简单的方法用于通过使用使上的羧化的多壁碳纳米管（MWCNT-COOH）和羧化的石墨烯纳米片（GR-COOH）曼赫根毛霉脂肪酶（RML）的生物缀合和固定胩基四组分反应。在这种方法中，酶提供氨基，支撑件供应羧酸基团，并将缺失的组分（异氰化物和醛）加入到反应介质中。该偶联反应在25℃下在水中进行，其中达到快速和高酶负载。对于GR-COOH和MWCNT-COOH，获得了530mg和680mg的最大负载能力。使用包括FTIR，拉曼光谱，XRD，SEM和TGA在内的各种技术来表征RML的固定衍生物。与可溶性酶相比，固定化制剂显示出显着增加的热稳定性和共溶剂稳定性。还测定了RML的动力学参数和最佳pH活性及其固定化制剂。为MWCNTS-RML，GR-RML和免费RML获得0.44,0.23和0.18mm的KM值，最大反应速率（V-MAX）为0.09,0.1和0.08 mm min（-1） 。该方法可以提供一种通用和有效的方法，以在环境条件下将生物分子附着在各种羧化固体表面上。

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  • 来源
    《RSC Advances》 |2016年第76期|共11页
  • 作者

    Mohammadi Mehdi; Ashjari Maryam; Garmroodi Maryam; Yousefi Maryam; Karkhane Ali Asghar;

  • 作者单位

    NIGEB Inst Ind &

    Environm Biotechnol Bioproc Engn Dept POB 14965-161 Tehran Iran;

    NIGEB Inst Ind &

    Environm Biotechnol Bioproc Engn Dept POB 14965-161 Tehran Iran;

    Univ Zanjan Dept Chem Zanjan Iran;

    ACECR Avicenna Res Inst Nanobiotechnol Res Ctr Tehran Iran;

    NIGEB Inst Ind &

    Environm Biotechnol Syst Biotechnol Dept Tehran Iran;

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  • 正文语种 eng
  • 中图分类 化学;
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