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Preparation and characterization of calcium phosphate/pectin scaffolds for bone tissue engineering

机译：骨组织工程磷酸钙/果胶支架的制备与表征

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摘要

A calcium phosphate cement (CPC) scaffold has been used to repair bone defects, but its low compressive strength and poor osteogenesis greatly hinder its clinical application. Pectin is a natural, biocompatible, biodegradable, water-soluble polysaccharide that can improve the osteogenic activity of biomaterials. In this study, calcium phosphate/pectin cement (CPCP) scaffolds with different pectin concentrations (2-6%) were prepared. The results showed that changes in the pectin concentration could modulate the morphology of the CPC scaffold. Moreover, CPCP scaffolds can significantly improve the compressive strength of the CPC. For example, the compressive strength reached 28.76 MPa when the concentration of pectin was 6%, which is similar to the mechanical strength of cancellous bone. In addition, the effects of the CPCP scaffolds on cell behaviour were investigated using a model of human adipose-derived stem cells (hADSCs). Our data showed that the introduction of pectin promoted the attachment and proliferation of hADSCs in vitro. Moreover, hADSCs seeded onto the CPCP scaffold showed higher osteogenetic efficacy than those seeded onto the CPC scaffold by modulating the expression of osteogenic genes. Based on these results, a pre-clinical study was performed in New Zealand white rabbits using the cavity defect model. Furthermore, new bone could be observed surrounding the CPCP scaffold within 8 weeks. These results indicate that the CPCP scaffold has potential applications in repairing bone defects.
机译：磷酸钙水泥（CPC）支架已经用于修复骨缺损，但其低压缩强度和差的成骨发生极大地阻碍了其临床应用。果胶是一种天然，生物相容性，可生物降解的水溶性多糖，可以改善生物材料的骨质发生活性。在该研究中，制备具有不同果胶浓度（2-6％）的磷酸钙/果胶水泥（CPCP）支架。结果表明，果胶浓度的变化可以调节CPC支架的形态。此外，CPCP支架可以显着提高CPC的抗压强度。例如，当果胶浓度为6％时，压缩强度达到28.76MPa，其类似于松质骨的机械强度。此外，使用人脂肪衍生的干细胞（HADSCs）的模型研究了CPCP支架对细胞行为的影响。我们的数据表明，果胶引入促进了体外哈姆斯菌株的附着和增殖。此外，通过调节成骨基因的表达，培养到CPCP支架上的HADSCs比种子溶血基团上的骨发生效果较高。基于这些结果，使用腔缺陷模型在新西兰白兔中进行临床前研究。此外，可以在8周内围绕CPCP支架观察新骨。这些结果表明CPCP支架具有修复骨缺损的潜在应用。

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  • 来源
    《RSC Advances》 |2016年第67期|共12页
  • 作者

    Zhao Lisheng; Li Junjie; Zhang Liang; Wang Yu; Wang Jiexin; Gu Bin; Chen Jianfeng; Hao Tong; Wang Changyong; Wen Ning;

  • 作者单位

    Chinese Peoples Liberat Army Gen Hosp Dept Stomatol Beijing 100853 Peoples R China;

    Acad Mil Med Sci Inst Basic Med Sci Dept Adv Interdisciplinary Studies 27 Taiping Rd Beijing 100850 Peoples R China;

    Gen Hosp Beijing Mil Reg Out Patient Dept 2 Beijing 100125 Peoples R China;

    Chinese Peoples Liberat Army Gen Hosp Dept Stomatol Beijing 100853 Peoples R China;

    Beijing Univ Chem Technol Key Lab Nanomat Minist Educ Beijing 100029 Peoples R China;

    Chinese Peoples Liberat Army Gen Hosp Dept Stomatol Beijing 100853 Peoples R China;

    Dalian Med Univ Affiliated Hosp 1 Dept Prosthodont Dalian 116011 Peoples R China;

    Acad Mil Med Sci Inst Basic Med Sci Dept Adv Interdisciplinary Studies 27 Taiping Rd Beijing 100850 Peoples R China;

    Acad Mil Med Sci Inst Basic Med Sci Dept Adv Interdisciplinary Studies 27 Taiping Rd Beijing 100850 Peoples R China;

    Chinese Peoples Liberat Army Gen Hosp Dept Stomatol Beijing 100853 Peoples R China;

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