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FePt nanoparticles: a novel nanoprobe for enhanced HeLa cells sensitivity to chemoradiotherapy

机译：纳米粒子：一种用于增强的Hela细胞对化学疗法的敏感性的新植物

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摘要

Chemoradiotherapy is a well-established treatment paradigm in oncology to consistently improve local tumor control compared to the sole administration of chemotherapy or radiotherapy. Despite its importance, few agents have been identified to further improve the therapeutic ratio and reduce the incidence of complications. Advances in nanomedicine have offered innovative strategies to improve chemoradiotherapy. In the present study, we evaluated the efficacy and safety of FePt nanoparticles (NPs) in chemoradiotherapy using HeLa cells and HEK293T cells. FexPt100-x NPs at different compositions (x = 26, 53 and 77) were synthesized and characterized by means of X-ray diffraction, transmission electron microscopy, and Fourier transform infrared spectroscopy. After ligand exchange, the cytotoxicity of FexPt100-x NPs was evaluated by MTT assay in HEK293T cells while the composition effects of FePt NPs on the cytotoxicity and the potential application of FePt NPs in combination with Xray radiation at clinically relevant MV energies were investigated in HeLa cells in vitro. Besides, the cellular uptake of NPs was measured indirectly by atomic absorption spectroscopy and transmission electron microscopy. The results indicated that FexPt100-x NPs inhibited the growth of HeLa cells in a concentration-and composition-dependent manner after 24 h incubation, with low cytotoxicity to HEK293T cells at the given concentrations (0-20 mu g mL(-1)). Furthermore, the combination of FePt NPs and radiotherapy resulted in a marked inhibition of HeLa cells, in contrast with that of the individual FePt NPs treated group or the radiation alone group. Moreover, Fe53Pt47 NPs, exhibiting significant cytotoxicity and enhanced radiosensitization effects on HeLa cells without damage to HEK293T cells, might theoretically satisfy the ultimate goal of personalized chemoradiotherapy. Our present work exhibited high therapeutic efficacy of FePt NPs in combination with radiotherapy without apparent cytotoxicity, suggesting the potential of FePt NPs as a promising nanoprobe in improving the outcome of tumor chemoradiotherapy.
机译：与唯一的化疗或放射疗法相比，化学疗法是肿瘤中核查的良好治疗范式，以始终如一地改善局部肿瘤控制。尽管重要的是，已经确定了很少的药剂以进一步提高治疗比率，降低并发症的发生率。纳米医生的进展提供了提高化学疗法的创新策略。在本研究中，我们使用Heha细胞和HEK293T细胞评估了备态纳米粒子（NPS）在化学疗法中的疗效和安全性。通过X射线衍射，透射电子显微镜和傅里叶变换红外光谱合成并表征不同组合物（X = 26,53和77）的FEXPT100-X NPS。在配体交换之后，通过HEK293T细胞中的MTT测定评估FEXPT100-X NPS的细胞毒性，同时在HELA中研究了对临床相关的MV能量的X射线辐射结合X射线辐射的细胞毒性和潜在的NPS潜在应用的组成作用。细胞体外。此外，通过原子吸收光谱和透射电子显微镜间接测量NPS的细胞吸收。结果表明FEXPT100-X NPS在24小时孵育后抑制HeLa细胞以浓度 - 和组成依赖性方式的生长，在给定浓度下对HEK293T细胞的低细胞毒性（0-20μg（-1）） 。此外，备用NPS和放射疗法的组合导致Hela细胞的明显抑制，与单独的NPS处理基团或辐射单独组的相比之下。此外，Fe53PT47 NPS，表现出显着的细胞毒性和增强对HeLa细胞的放射敏化作用，而不会损坏HEK293T细胞，可能理论地满足个性化化学疗法的最终目标。我们目前的工作表现出备用NP与无放射疗法的高治疗效果，没有表观细胞毒性，表明备用NPS作为提高肿瘤化学疗法的结果的纳米孔的潜力。

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  • 来源
    《RSC Advances》 |2016年第41期|共11页
  • 作者

    Bao Zhirong; He Mingyang; Quan Hong; Jiang Dazhen; Zheng Yanhong; Qin Wenjing; Zhou Yunfeng; Ren Feng; Guo Mingxiong; Jiang Changzhong;

  • 作者单位

    Wuhan Univ Minist Educ Key Lab Artificial Micro &

    Nanostruct Wuhan 430072 Peoples R China;

    Wuhan Univ Coll Life Sci Wuhan 430072 Peoples R China;

    Wuhan Univ Minist Educ Key Lab Artificial Micro &

    Nanostruct Wuhan 430072 Peoples R China;

    Wuhan Univ Minist Educ Key Lab Artificial Micro &

    Nanostruct Wuhan 430072 Peoples R China;

    Huazhong Univ Sci &

    Technol Tongji Med Coll Tongji Hosp Oncol Dept Huazhong Peoples R China;

    Wuhan Univ Minist Educ Key Lab Artificial Micro &

    Nanostruct Wuhan 430072 Peoples R China;

    Wuhan Univ Zhongnan Hosp Dept Radiat &

    Med Oncol Wuhan 430072 Peoples R China;

    Wuhan Univ Minist Educ Key Lab Artificial Micro &

    Nanostruct Wuhan 430072 Peoples R China;

    Wuhan Univ Coll Life Sci Wuhan 430072 Peoples R China;

    Wuhan Univ Minist Educ Key Lab Artificial Micro &

    Nanostruct Wuhan 430072 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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