首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the L,D-transpeptidase LdtMt1 from Mycobacterium tuberculosis
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Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the L,D-transpeptidase LdtMt1 from Mycobacterium tuberculosis

机译:结核分枝杆菌L,D-转肽酶LdtMt1的表达,纯化,结晶和初步X射线晶体学分析

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摘要

Mycobacterium tuberculosis is capable of adapting to prolonged periods of dormancy, a state which is resistant to killing by antimycobacterial agents. The L,D-transpeptidation reaction catalysed by the L,D-transpeptidase LdtMt1 is likely to play an essential role in the adaptation of M. tuberculosis to its dormant state. LdtMt1 has been successfully crystallized using vapour-diffusion methods. The crystals of this protein belonged to space group P6522, with unit-cell parameters a = 57.25, b = 57.25, c = 257.96 angstrom, = 90, = 90, = 120 degrees. Diffraction data have also been collected from a selenomethionine derivative to 2.9 angstrom resolution. Model building using the phases derived from the multiwavelength anomalous dispersion experiment is in progress.
机译:结核分枝杆菌能够适应延长的休眠期,这种休眠期对抗分枝杆菌药的杀灭具有抵抗力。由L,D-转肽酶LdtMt1催化的L,D-转肽反应很可能在结核分枝杆菌适应其休眠状态中起重要作用。 LdtMt1已成功地使用蒸汽扩散法结晶。该蛋白质的晶体属于空间群P6522,单位细胞参数a = 57.25,b = 57.25,c = 257.96埃,= 90,= 90,= 120度。衍射数据也已经从硒代蛋氨酸衍生物收集到2.9埃的分辨率。正在使用从多波长异常色散实验得出的相位建立模型。

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