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首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Crystallization and preliminary X-ray diffraction analysis of a DING protein from Pseudomonas aeruginosa PA14
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Crystallization and preliminary X-ray diffraction analysis of a DING protein from Pseudomonas aeruginosa PA14

机译：铜绿假单胞菌PA14中DING蛋白的结晶和初步X射线衍射分析

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摘要

DING proteins form an emergent family of proteins consisting of an increasing number of homologues that have been identified in all kingdoms of life. They belong to the superfamily of phosphate-binding proteins and exhibit a high affinity for phosphate. In eukaryotes, DING proteins have been isolated by virtue of their implication in several diseases and biological processes. Some of them are potent inhibitors of HIV-1 replication/transcription, raising the question of their potential involvement in the human defence system. Recently, a protein from Pseudomonas aeruginosa strain PA14, named PA14DING or LapC, belonging to the DING family has been identified. The structure of PA14DING, combined with detailed biochemical characterization and comparative analysis with available DING protein structures, will be helpful in understanding the structural determinants implicated in the inhibition of HIV-1 by DING proteins. Here, the expression, purification and crystallization of PA14DING and the collection of X-ray data to 1.9 angstrom resolution are reported.
机译：DING蛋白质形成了一个新兴的蛋白质家族，其中包括越来越多的同源物，这些同源物已在所有生命王国中被发现。它们属于磷酸盐结合蛋白的超家族，对磷酸盐显示出高亲和力。在真核生物中，由于DING蛋白与多种疾病和生物学过程有关，因此已分离出DING蛋白。其中一些是HIV-1复制/转录的有效抑制剂，这提出了它们可能参与人类防御系统的问题。最近，已经鉴定出属于DING家族的铜绿假单胞菌菌株PA14的一种名为PA14DING或LapC的蛋白质。 PA14DING的结构，结合详细的生化特征和可利用的DING蛋白质结构的比较分析，将有助于理解与DING蛋白质抑制HIV-1有关的结构决定因素。在此，报道了PA14DING的表达，纯化和结晶以及以1.9埃分辨率的X射线数据的收集。

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