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首页> 外文期刊>Biochemical Genetics >Combination of Simple Sequence Repeat, S-Locus Polymorphism and Phenotypic Data for Identification of Tunisian Plum Species (Prunus spp.)
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Combination of Simple Sequence Repeat, S-Locus Polymorphism and Phenotypic Data for Identification of Tunisian Plum Species (Prunus spp.)

机译：简单序列重复，S-轨迹多态性和表型数据的组合凝视突尼斯梅（Prunus SPP）

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摘要

Plums (Prunus spp.) are among the first fruit tree species that attracted human interest. Artificial crosses between wild and domesticated species of plums are still paving the way for creation of new phenotypic variability. In Tunisia, despite a considerable varietal richness of plum as well as a high economic value, the plum sector is experiencing a significant regression. The main reason of this regression is the absence of a national program of plum conservation. Hence, this work was aimed to phenotypically and genetically characterize 23 Tunisian plum accessions to preserve this patrimony. Closely related Prunus species from the same subgenus may be differing at two characteristics: ploidy level and phenotypic traits. In this study, single sequence repeat (SSR) markers allowed distinguishing between eighteen diploid accessions and five polyploid accessions, but SSR data alone precluded unambiguous ploidy estimation due to homozygosity. In contrast, S-allele markers were useful to identify the ploidy level between polyploid species, but they did not distinguish species with the same ploidy level. Seven out of 12 phenotypic traits were shown to be discriminant traits for plum species identification. Molecular and phenotypic traits were significantly correlated and revealed a powerful tool to draw taxonomic and genotypic keys. The results obtained in this work are of great importance for local Tunisian plum germplasm management.
机译：李子（Prunus spp。）是吸引人类兴趣的第一家果树等物种之一。野生和驯养物种之间的人工交叉仍然为创造新的表型变异性铺平了途径。在突尼斯，尽管梅花的丰富性相当大，但经济价值很高，梅花部门正在经历重大回归。这种回归的主要原因是没有国家梅花保护计划。因此，这项工作旨在表型和基因表征23个突尼斯梅花泄露以保护这种遗产。来自同一子根属的密切相关的蛋白质可能是不同的两个特征：倍率水平和表型性状。在本研究中，单序重复（SSR）标记允许区分18个二倍体摘录和五个多倍体载体，但是由于纯合子而单独的SSR数据排除明确的倍数梯级估计。相比之下，S-等位基因标记可用于鉴定多倍体物种之间的倍倍率水平，但它们没有区分物种具有相同的倍性水平。 12个表型特征中的七种表现为歧视物种鉴定的判别性状。分子和表型性状显着相关，并揭示了绘制分类学和基因型键的强大工具。在本工作中获得的结果对于当地突尼斯梅子种质管理具有重要意义。

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