HIV-integrase aptamer folds into a parallel quadruplex: a thermodynamic study.

Kelley S; Boroda S; Musier Forsyth K; Kankia BI

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HIV-integrase aptamer folds into a parallel quadruplex: a thermodynamic study.

机译：HIV-整合酶适体折叠成平行的QuadRuplex：热力学研究。

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Short guanine-rich sequences have a tendency to form quadruplexes that are stabilized by G-quartets with specific cation coordination. Quadruplexes are part of telomeres at the ends of chromosomes and play an important role in the regulation of gene expression. In addition, there is a strong interest in the therapeutic and biotechnological potential of quadruplex oligonucleotides. The HIV-integrase aptamer, d(GGGT)(4), demonstrated unusually favorable van't Hoff thermodynamics, and based on NMR studies the aptamer was proposed to fold into an antiparallel structure. Here we probed an apparent discrepancy between the NMR structure and the quadruplex topology suggested by circular dichroism (CD). Systematic thermodynamic analyses of d(GGGT)(4) and variants containing sequence modifications or missing specific nucleotides are consistent with a parallel quadruplex fold. CD studies carried out over a wide concentration range did not support a possible structural transition upon increasing strand concentration. Taken together, both optical and thermodynamic studies performed here strongly support a parallel fold for the d(GGGT)(4) aptamer.

机译：富含短鸟嘌呤的序列具有形成通过具有特定阳离子协调的G-quartets稳定的四边形的四核。四氟酯是染色体末端端粒的一部分，并在基因表达的调节中起重要作用。此外，对四氢寡核苷酸的治疗和生物技术潜力存在浓厚的兴趣。 HIV-整合酶适体，D（GGGT）（4）表现出异常有利的VACOR HOFF热力学，并且基于NMR研究，拟议适体折叠成反平行结构。在这里，我们探讨了NMR结构与圆形二色性（CD）建议的四边形拓扑之间的表观差异。 D（GGGT）（4）和含有序列修饰或缺失的特定核苷酸的变体的系统热力学分析与平行的四边形折叠一致。在宽浓度范围内进行的CD研究在增加股线浓度时不支持可能的结构转变。连同，在此进行的光学和热力学研究均强烈地支持D（GGGT）（4）适体的平行折叠。

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来源
《Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena》 |2011年第3期|共7页
作者
Kelley S; Boroda S; Musier Forsyth K; Kankia BI;
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Department of Chemistry The Ohio State University Columbus OH 43210 USA.;

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正文语种 eng
中图分类生物化学;
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1. HIV-integrase aptamer folds into a parallel quadruplex: a thermodynamic study. [J] . Kelley S, Boroda S, Musier Forsyth K, Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena . 2011,第2a3期

机译：HIV整合酶适体折叠成平行四链体：热力学研究。
2. HIV-integrase aptamer folds into a parallel quadruplex: a thermodynamic study. [J] . Kelley S, Boroda S, Musier Forsyth K, Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena . 2011,第2a3期

机译：HIV-整合酶适体折叠成平行的QuadRuplex：热力学研究。
3. HIV-integrase aptamer folds into a parallel quadruplex: a thermodynamic study. [J] . Kelley S, Boroda S, Musier Forsyth K, Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena . 2011,第2a3期

机译：HIV-整合酶适体折叠成平行的QuadRuplex：热力学研究。
4. Thermodynamics and kinetics study of individual K~+ binding events reveal new aspects of DNA G-quadruplex folding pathways [C] . Adrien Marchand, Valerie Gabelica ASMS Conference on Mass Spectrometry and Allied Topics . 2015

机译：单个K〜+结合事件的热力学和动力学研究揭示了DNA G-Quadruple折叠途径的新方面
5. Nonenzymatic oligomerization of RNA by TNA templates, and, Isoguanine-modified thrombin aptamers, and, An antiparallel purine·purine DNA duplex, and, Synthesis and enzymatic polymerization of FNA on DNA templates, and, Thermodynamics and luminescence of novel metallo-DNA base-pairs. [D] . Heuberger, Benjamin David. 2007

机译：TNA模板，异鸟嘌呤修饰的凝血酶适体，反平行嘌呤·嘌呤DNA双链体以及FNA在DNA模板上的合成和酶促聚合，以及新型金属DNA碱基的热力学和发光，通过RNA的非酶促寡聚化对。
6. Thermodynamic stability and folding kinetics of the major G-quadruplex and its loop-isomers formed in the Nuclease Hypersensitive Element in the human c-Myc promoter-Effect of loops and flanking segments on the stability of parallel-stranded intramolecular G-quadruplexes [O] . Emmanuel Hatzakis, Keika Okamoto, Danzhou Yang -1

机译：主要G-quadruplex的热力学稳定性和折叠动力学及其在核酸酶的核酸酶的过敏元件中形成的环状异构体在LOOPS和侧翼段对平行链分子内g-四翻来的稳定性的影响
7. HIV-Integrase Aptamer Folds into a Parallel Quadruplex: A Thermodynamic Study [O] . Kelly Sean, Boroda Salome, Musier-Forsyth Karin, 2011

机译：HIV整合适体折叠成平行四重体：热力学研究。

HIV-integrase aptamer folds into a parallel quadruplex: a thermodynamic study.

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