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首页> 外文期刊>Cytokine >Detection of TH1/TH2 cytokine signatures in yellow fever 17DD first-time vaccinees through ELISpot assay.
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Detection of TH1/TH2 cytokine signatures in yellow fever 17DD first-time vaccinees through ELISpot assay.

机译：通过ELISpot分析检测黄热病17DD初次接种疫苗中TH1 / TH2细胞因子的特征。

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摘要

Immunity to yellow fever (YF) is conferred by the interplay of humoral and cellular immune response. Despite the extensive literature on the humoral immune response to the YF vaccine virus, little is known about its cellular immune response to vaccination. The analysis of cytokine production by ex-vivo antigen-stimulated T cells has been considered as a valuable tool for understanding cellular immune response. Thus, we have analyzed two T(H)1/T(H)2 signature cytokines (IFN-gamma and IL-4) from 12 healthy first-time adults vaccinated with YF17DD virus. The cells, harvested on day 0 (before vaccination) and 7, 15 and 30 days after immunization were antigen-stimulated and analyzed by ELISpot. A significant increase in the number of spot-forming cells during the response to YF 17DD live virus stimulation by ELISpot assay was observed. IFN-gamma-and IL-4-producing cells were significantly increased on the 15th day after vaccination in all volunteers. These results presented herein are important for understanding the role of cytokines in the immune response to YF 17DD virus.
机译：体液和细胞免疫反应的相互作用赋予了黄热病（YF）免疫力。尽管有关YF疫苗病毒的体液免疫反应的文献很多，但对其对疫苗的细胞免疫反应知之甚少。体外抗原刺激的T细胞对细胞因子产生的分析被认为是了解细胞免疫反应的有价值的工具。因此，我们分析了来自接种YF17DD病毒的12名健康初次成年成年人的两种T（H）1 / T（H）2标志性细胞因子（IFN-γ和IL-4）。抗原刺激并在第0天（接种前）和免疫后7、15和30天收获的细胞进行了抗原分析，并通过ELISpot进行了分析。观察到通过ELISpot分析对YF 17DD活病毒刺激的反应过程中斑点形成细胞的数量显着增加。接种疫苗后第15天，所有志愿者的IFN-γ和IL-4产生细胞均显着增加。本文呈现的这些结果对于理解细胞因子在针对YF 17DD病毒的免疫反应中的作用非常重要。

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