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Further characterisation of cytokines in macropod marsupials: IL-10 and IL-10 Delta 3

机译：大脚有袋动物中细胞因子的进一步表征：IL-10和IL-10 Delta 3

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Interleukin-10 is an immunomodulatory cytokine that has been implicated, along with IFN-gamma, in the disease sequelae of mycobacterial infection. In order to investigate the role of IL-10 in marsupial disease models we sequenced and characterised the IL10 gene in the tammar wallaby (Macropus eugenii) and ruf ous hare-wallaby (Lagorchestes hirsutus). An isoform IL-10 Delta 3, in which an in-frame deletion of exon 3 occurs, was discovered in both macropod species. Analysis of wallaby and other reported marsupial IL-10 homologs suggests that while marsupial IL-10 is comparable to that of human IL-10, the predicted IL-10 Delta 3 protein may play a more complicated role in the modulation of 1-10-directed responses. Expression of the canonical gene and splicing variant was confirmed in both wallabies, and the rufous hare-wallaby showed differential expression across lymph node, spleen and liver, with isoform expression detected in the lymph node. This characterisation and expression of IL-10 in de novo tissues provides a basis for further study into the role of IL-10 in disease models in marsupials. (C) 2016 Elsevier Ltd. All rights reserved.
机译：白介素10是一种免疫调节性细胞因子，已与IFN-γ一起参与了分枝杆菌感染的疾病后遗症。为了研究IL-10在有袋动物疾病模型中的作用，我们在淡色小袋鼠（Macropus eugenii）和棕兔-小袋鼠（Lagorchestes hirsutus）中对IL10基因进行了测序和鉴定。在两个大型足类物种中均发现了同种型IL-10 Delta 3，其中发生了外显子3的框内缺失。对小袋鼠和其他​​报道的有袋动物IL-10同源物的分析表明，尽管有袋动物IL-10与人IL-10相当，但预测的IL-10 Delta 3蛋白可能在1-10-的调节中起更复杂的作用直接回应。在两个小袋鼠中都证实了规范基因和剪接变体的表达，而红褐色野鼠在淋巴结，脾脏和肝脏中表现出差异表达，并且在淋巴结中检测到同工型表达。从头组织中IL-10的这种表征和表达为进一步研究IL-10在有袋动物疾病模型中的作用提供了基础。 （C）2016 Elsevier Ltd.保留所有权利。

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