• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical microbiology and infection: European Society of Clinical Microbiology and Infectious Diseases >Detection of Streptococcus pneumoniae colonisation in respiratory tract secretions of military personnel.
【24h】

Detection of Streptococcus pneumoniae colonisation in respiratory tract secretions of military personnel.

机译：军事人员呼吸道分泌物中肺炎链球菌定殖的检测。

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Respiratory tract infections with Streptococcus pneumoniae are an important cause of morbidity and mortality among military personnel. A sensitive method is needed to determine the prevalence of S. pneumoniae colonisation in respiratory secretions, as well as its role in pneumonia without an established aetiology. This study investigated the efficacy of two PCR assays in screening military personnel for S. pneumoniae colonisation. Nasopharyngeal swabs were obtained from 200 military personnel and tested for S. pneumoniae by culture and PCR. S. pneumoniae was cultured from three (1.5%) of the 200 samples. PCR for the lytA gene detected S. pneumoniae in 11% of the samples, while PCR for the pneumolysin gene detected S. pneumoniae in 3% of the samples. The sensitivity and negative predictive values were 100% for both PCR assays when compared to culture; the specificity and positive predictive values for the lytA PCR were 90.4% and 13.6%, respectively, compared with 98.5% and 50%, respectively, for the pneumolysin gene PCR. It was concluded that respiratory tract colonisation of military personnel with S. pneumoniae can be identified rapidly and reliably by PCR assays. The use of this technique may greatly enhance the ability to identify a microbial aetiology for pneumonia when compared with conventional culture methods.
机译：肺炎链球菌呼吸道感染是军事人员发病率和死亡率的重要原因。需要一种敏感的方法来确定呼吸分泌中S.肺炎肺炎的患病率，以及其在肺炎中的作用，没有建立的缓解学。本研究研究了两种PCR测定在肺炎肺炎殖民化军事人员中的疗效。鼻咽拭子是从200名军事人员获得的，并通过培养和PCR对S.肺炎进行测试。肺炎肺炎从200个样品中的三个（1.5％）培养。 Lyta基因的PCR在11％的样品中检测到S.肺炎。在3％的样品中检测到肺炎肠道基因的PCR。与培养相比，PCR测定的敏感性和阴性预测值为100％; Lyta PCR的特异性和阳性预测值分别为90.4％和13.6％，分别为98.5％和50％，分别用于肺炎蛋白基因PCR。得出结论是，通过PCR测定，可以通过PCR测定迅速可靠地识别肺炎的军事人员的呼吸道殖民。与常规培养方法相比，这种技术的使用可能大大提高鉴定肺炎的微生物缓解学的能力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号