• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Annual review of cell and developmental biology >Low-Affinity Binding Sites and the Transcription Factor Specificity Paradox in Eukaryotes
【24h】

Low-Affinity Binding Sites and the Transcription Factor Specificity Paradox in Eukaryotes

机译：低亲和力结合位点和真核生物中的转录因子特异性悖论

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Eukaryotic transcription factors (TFs) from the same structural family tend to bind similar DNA sequences, despite the ability of these TFs to execute distinct functions in vivo.The cell partly resolves this specificity paradox through combinatorial strategies and the use of low-affinity binding sites, which are better able to distinguish between similar TFs.However, because these sites have low affinity, it is challenging to understand how TFs recognize them in vivo.Here, we summarize recent findings and technological advancements that allow for the quantification and mechanistic interpretation of TF recognition across a wide range of affinities.We propose a model that integrates insights from the fields of genetics and cell biology to provide further conceptual understanding of TF binding specificity.We argue that in eukaryotes, target specificity is driven by an inhomogeneous 3D nuclear distribution of TFs and by variation in DNA binding affinity such that locally elevated TF concentration allows low-affinity binding sites to be functional.
机译：来自相同结构家族的真核转录因子（TFS）倾向于结合类似的DNA序列，尽管这些TFS在体内执行不同的功能的能力。细胞部分通过组合策略和使用低亲和力结合位点来解决这种特异性悖论，这更好地能够区分类似的TFS。然而，由于这些网站具有低亲和力，因此了解TFS如何在体内识别出来有挑战性。在内，我们概述了最近的发现和技术进步，允许对量化和机械解释的最新发现和技术进步TF识别各种亲和力。我们提出了一种模型，该模型将来自遗传和细胞生物领域的洞察力集成，以提供对TF结合特异性的进一步概念理解。我们认为在真核生物中，目标特异性是由不均匀的3D核分布驱动的TFS和DNA结合亲和力的变异，使得局部升高的TF浓度趋势允许低亲和力结合位点是功能性的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号