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Antibacterial activity and mechanism of green tea polysaccharide conjugates against Escherichia coli

机译：青茶蛋白缀合物对大肠杆菌的抗菌活性及机制

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摘要

The effect of green tea polysaccharide conjugate (gTPC) on the growth of E. coli was evaluated. gTPC has optimal antibacterial concentrations for E. coli DH5 alpha (4.00 mg/mL) and 25,922 (1.00 mg/mL), with the antibacterial rates of 55.75 % and 50.65 % respectively. The results of scanning electron microscopy (SEM) and transmission electron microscope (TEM) indicated that gTPC-treated E. coli cells showed obvious morphological changes, part of the cell wall became blurred, and the leakage of cytoplasmic content were observed. The proportion of the propidium iodide (PI) permeable E. coli cells treated using 0.50-2.00 mg/mL gTPC was between 8.79 %-9.49 % as well as 14.10 % (4.00 mg/mL gTPC) and 15.10 % (6.00 mg/mL gTPC). Reactive oxygen species (ROS) production of 1.00-4.00 mg/mL gTPC treated E. coli increased by 26.57 %-38.19 % with a significant increase level (p < 0.01). The outer membrane disruption played a key role in the antibacterial activity of gTPC against E. coli. This research result will open a new application field of tea polysaccharide conjugates.
机译：评估绿茶多糖缀合物（GTPC）对大肠杆菌生长的影响。 GTPC具有大肠杆菌DH5α（4.00mg / ml）和25,922（1.00mg / ml）的最佳抗菌浓度，抗菌率分别为55.75％和50.65％。扫描电子显微镜（SEM）和透射电子显微镜（TEM）的结果表明GTPC处理的大肠杆菌细胞显示出明显的形态变化，部分细胞壁变得模糊，观察到细胞质含量的泄漏。使用0.50-2.00mg / ml GTPC处理的碘化钛（PI）可渗透的大肠杆菌细胞的比例为8.79％-9.49％以及14.10％（4.00mg / ml GTPC）和15.10％（6.00mg / ml gtpc）。反应性氧物种（ROS）产生1.00-4.00mg / ml GTPC处理的大肠杆菌，大肠杆菌增加了26.57％-38.19％，水平显着增加（P <0.01）。外膜破坏在GTPC对大肠杆菌的抗菌活性中发挥了关键作用。该研究结果将打开茶多糖缀合物的新应用领域。

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  • 来源
    《Industrial Crops and Products》 |2020年第1期|共8页
  • 作者

    Zhou Yin; Yao Qifeng; Zhang Tong; Chen Xiaoqiang; Wu Zhengqi; Zhang Ni; Shao Yundong; Cheng Yong;

  • 作者单位

    Hubei Univ Technol Natl Ctr Cellular Regulat &

    Mol Pharmaceut 111 Key Lab Fermentat Engn Minist Educ Wuhan 430068 Peoples R China;

    Hubei Univ Technol Natl Ctr Cellular Regulat &

    Mol Pharmaceut 111 Key Lab Fermentat Engn Minist Educ Wuhan 430068 Peoples R China;

    Hubei Univ Technol Natl Ctr Cellular Regulat &

    Mol Pharmaceut 111 Key Lab Fermentat Engn Minist Educ Wuhan 430068 Peoples R China;

    Hubei Univ Technol Natl Ctr Cellular Regulat &

    Mol Pharmaceut 111 Key Lab Fermentat Engn Minist Educ Wuhan 430068 Peoples R China;

    Hubei Univ Technol Natl Ctr Cellular Regulat &

    Mol Pharmaceut 111 Key Lab Fermentat Engn Minist Educ Wuhan 430068 Peoples R China;

    Huazhong Univ Sci &

    Technol Tongji Hosp Dept Thorac Surg Wuhan 430030 Hubei Peoples R China;

    Zhejiang Skyherb Biotechnol Inc Huzhou 313300 Peoples R China;

    Zhejiang Skyherb Biotechnol Inc Huzhou 313300 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 农作物;
  • 关键词

    Tea polysaccharide conjugate; Antibacterial activity; Escherichia coli; Cell membrane;

    机译：茶多糖缀合物;抗菌活性;大肠杆菌;细胞膜;

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