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首页> 外文期刊>International journal of computerized dentistry >Attachment and growth of human osteoblasts on different biomaterial surfaces
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Attachment and growth of human osteoblasts on different biomaterial surfaces

机译：人骨盆对不同生物材料表面的附着和生长

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Objectives: To prove the biocompatibility of biomaterials applied in biomedical devices, in vitro testing is crucial to render a material fit for medical application. The material of choice for dental implants is commercially pure titanium (cp-Ti), while other materials such as zirconia and polyetherether-ketone (PEEK) are considered highly promising due to their functional and esthetic properties. The aim of this study was to determine whether PEEK with defined mean surface roughness and composition could achieve results equal to titanium or zirconia. Materials and methods: Disks measuring 14 mm in diameter and 1 mm in thickness made from cp-Ti, yttria-stabilized zirconia (Y-TZP), and filled PEEK with a smooth surface finish were used for cell culture experiments. Human fetal osteoblasts (hFOB) were cultured in vitro on each material to observe changes after 1,3, and 7 days regarding cell viability and lactate dehydrogenase (LDH) release. Additionally, mRNA expression of proliferative factors PCNA and Ki67 and cellular adhesion (vinculin mRNA expression and immunoflu-orescence staining) were analyzed after 3 days in the culture. Results: In hFOB cultures, adhesion and viability were decreased on PEEK platelets, while LDH release remained stable. No significant difference was observed in cp-Ti and Y-TZP when compared to the control. Conclusions: The performance of cp-Ti and Y-TZP was equal to the control in all tests. It seems that highly polished PEEK in this particular composition cannot be recommended for osseointegrated implant applications due to decreased oste-oblast attachment. Further investigations are recommended, especially in surface structures optimized for osseointegra-tion.
机译：目的：为了证明在生物医学装置中应用的生物材料的生物相容性，在体外测试是至关重要的，使材料适合医学应用。牙科植入物的选择材料是商业纯钛（CP-TI），而其他材料如氧化锆和聚醚醚 - 酮（PEEK）被认为是由于其功能和美学性质的高度令人令人市遇。本研究的目的是确定具有定义的平均表面粗糙度和组合物的偷看是否可以实现等于钛或氧化锆的结果。材料和方法：用CP-Ti，ytTRIA稳定的氧化锆（Y-TZP）的直径为14mm的磁盘，厚度为1mm，并填充具有光滑表面光洁度的填充PEEK，用于细胞培养实验。在每种材料上体外培养人胎骨细胞（HFOB），以观察1,3后的变化，以及关于细胞活力和乳酸脱氢酶（LDH）释放的7天。另外，在培养3天后，在3天后分析了增殖因子PCNA和KI67和细胞粘附（Vinculin mRNA表达和免疫染色的细胞粘附（Vinculin mRNA表达和免疫染色）的mRNA表达。结果：在PEEK血小板上，在HFOB培养物中，粘附和活力降低，而LDH释放保持稳定。与对照相比，CP-TI和Y-TZP在CP-TI和Y-TZP中没有观察到显着差异。结论：CP-Ti和Y-TZP的性能等于所有测试中的控制。似乎由于大稳定性倾斜率下降，似乎不能建议在这种特定组合物中的垂直植入物应用。建议进一步调查，特别是在针对OsseoIntion的表面结构中。

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