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首页> 外文期刊>Food Control >Biosafety assessment of Bifidobacterium animalis subsp. lactis AD011 used for human consumption as a probiotic microorganism
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Biosafety assessment of Bifidobacterium animalis subsp. lactis AD011 used for human consumption as a probiotic microorganism

机译：双歧杆菌患者的生物安全评估。 Lactis Ad011用于人类消费作为益生菌微生物

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In this work, the previously identified Bifidobacterium strain, Bifidobacterium animalis subsp. lactis AD011 (obtained from an infant fecal sample), intended as a potential probiotic microorganism, is assessed for its safety using multiple in vitro virulence assays (ammonia and biogenic amine synthesis, hemolytic and mucin degradation activities, antimicrobial susceptibility and conjugal transferability of antibiotic resistance genes to other microorganisms) and comparative genomic analysis. The genome data of B. lactis AD011 was compared with genome sequences of two commercially available probiotic microorganisms (Bifidobacterium lactis BB-12 and Bifidobacterium lactis Bl-04) which are designated as generally recognized as safe by the U.S. Food and Drug Administration. The results of these experiments showed no significant potential vulnerabilities and no side effects. B. lactis AD011 showed higher resistance to tetracycline than the European Food Safety Authority cut-off. However, the measured susceptibility is similar to or lower than that of other previously certified GRAS Bifidobacterium strains. Tetracycline resistance of B. lactis AD011 was not conveyed via conjugation with L. fermentum AGBG1, which is susceptible to tetracycline. Complete DNA sequencing of B. lactis AD011 showed the absence of transferable drug resistance plasmids. The three B. lactis strains (AD011, BB12 and Bl-14) compared in this study were found to share very close genomic sequence homology (>99.8%). Therefore, based on this study, B. lactis AD011 appears to be a safe, bioactive, bifidobacterial food ingredient, starter culture, and/or probiotic microorganism for human health.
机译：在这项工作中，先前鉴定的双歧杆菌菌株，双歧杆菌毒蕈吸潜伏。含有潜在益生菌微生物的乳酸盐Ad011（从婴儿粪便样品中获得），用于使用多种体外毒力测定（氨和生物胺合成，溶血性敏感性和抗生素抗性的抗微生物敏感性和蛋白质可转移性来评估其安全性评估其安全性其他微生物的基因和比较基因组分析。 B. Lactis Ad011的基因组数据与两种市售的益生菌微生物（双歧杆菌BB-12和双歧杆菌BB-04）的基因组序列进行了比较，其被指定为通常认为通过美国食品和药物管理的安全性。这些实验的结果没有显着的潜在脆弱性和没有副作用。 B.乳酸乳裂AD011表现出对四环素的抗性高于欧洲食品安全管理局的截止。然而，测量的易感性类似于或低于其他先前认证的Glas双歧杆菌菌株的敏感性。 B.乳酸乳酸乳溶液抗性抗乳杆菌抗性通过与L.Fermentum AgBG1的缀合，脂肪酸乳溶液AD011的抗性。 B. Lactis Ad011的完全DNA测序显示不存在可转移的耐药质粒。发现该研究比较的三种B.乳酸菌株（AD011，BB12和BL-14）分享非常紧密的基因组序列同源性（> 99.8％）。因此，基于该研究，B.Lactis Ad011似乎是一种安全，生物活性，双歧杆菌食品成分，起始培养物和/或人类健康的益生菌微生物。

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