Redox potential of the heam c group in the quinocytochrome, lupanine hydroxylase, an enzyme located in the periplasm of a Pseudomonas sp.

David J. Hopper; Jerzy Rogozinski

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Redox potential of the heam c group in the quinocytochrome, lupanine hydroxylase, an enzyme located in the periplasm of a Pseudomonas sp.

机译：喹诺酮细胞色素，Lupanine羟化酶（位于假单胞菌属种的周质中的一种酶）中的heam c组的氧化还原电位。

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The quinocytochrome c, lupanine hydroxylase, was shown to be located in the periplasm of a Pseudomonas sp. The midpoint redox potential of the haem in the purified enzyme was measured by potentiometric titration and shown to be + 193 mV. PQQ was removed from the enzyme by isoelectric focusing to give inactive apoenzyme. This resulted in a shift in the midpoint redox potential of the haem to + 98 mV. Full activity was recovered by the addition of PQQ to apoenzyme that also restored the original potential.

机译：奎诺细胞色素c，Lupanine羟化酶，显示位于假单胞菌属的周质中。通过电位滴定法测量纯化酶中血红素的中点氧化还原电势，显示为+ 193 mV。通过等电聚焦从酶中除去PQQ，得到失活的脱辅酶。这导致血红素的中点氧化还原电势转变为+ 98 mV。通过向脱辅酶中添加PQQ恢复了全部活性，这也恢复了原始潜力。

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《Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology》 |1998年第0期|共5页
作者
David J. Hopper; Jerzy Rogozinski;
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正文语种 eng
中图分类生物化学;分子生物学;
关键词
cytochrome c; quinocytochrome; PQQ; redox potential; lupanine hydroxylase; reconstitution;

机译：细胞色素c喹啉细胞色素PQQ氧化还原电位鲁潘宁羟化酶重构;

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1. Redox potential of the heam c group in the quinocytochrome, lupanine hydroxylase, an enzyme located in the periplasm of a Pseudomonas sp. [J] . David J. Hopper, Jerzy Rogozinski Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology . 1998,第0期

机译：喹诺酮细胞色素，Lupanine羟化酶（位于假单胞菌属种的周质中的一种酶）中的heam c组的氧化还原电位。
2. Cloning, sequencing and heterologous expression of the gene for lupanine hydroxylase, a quinocytochrome c from a Pseudomonas sp. [J] . Hopper DJ, Kaderbhai MA, Marriott SA, The Biochemical Journal . 2002,第Pta2期

机译：Lupanine羟化酶基因的克隆，测序和异源表达，Pseudomonas sp。的一种quinocytochrome c。
3. Cloning, sequencing and heterologous expression of the gene for lupanine hydroxylase, a quinocytochrome c from a Pseudomonas sp. [J] . Hopper DJ, Kaderbhai MA, Marriott SA, The Biochemical Journal . 2002,第Pta2期

机译：Lupanine羟化酶基因的克隆，测序和异源表达，Pseudomonas sp。的一种quinocytochrome c。
4. isolation and identiifcation of lipase producing microorganism from a newly isolated pseudomonas sp. 8309 and some properties of crude enzyme [C] . Shouwen Chen, Rou Xu, Kechang Zhang Asia-Pacific biochemical engineering conference;APBIOCHEC'97 . 1997

机译：分离和鉴定新分离的假单胞菌属的产脂肪酶微生物。 8309和粗酶的一些特性
5. Characterisation of phenol hydroxylase and its auxiliary proteins, DmpM and DmpK, from Pseudomonas sp. strain CF600 [D] . Cadieux, Elisabeth 2002

机译：表征苯酚羟化酶及其辅助蛋白DmpM和DmpK，来自假单胞菌。 CF600菌株
6. Cloning sequencing and heterologous expression of the gene for lupanine hydroxylase a quinocytochrome c from a Pseudomonas sp. [O] . David J Hopper, Mustak A Kaderbhai, Shirley A Marriott, 2002

机译：Lupanine羟化酶基因的克隆测序和异源表达Pseudomonas sp。的一种quinocytochrome c。
7. Cloning, sequencing and heterologous expression of the gene for lupanine hydroxylase, a quinocytochrome c from a Pseudomonas sp. [O] . Young Michael, Rogozinski Jerry, Marriott Shirley A., 2002

机译：Lupanine羟化酶基因的克隆，测序和异源表达，Pseudomonas sp。的一种quinocytochrome c。

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Redox potential of the heam c group in the quinocytochrome, lupanine hydroxylase, an enzyme located in the periplasm of a Pseudomonas sp.

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