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首页> 外文期刊>Fish & Shellfish Immunology >Efficacy of live attenuated vaccine derived from the Streptococcus agalactiae on the immune responses of Oreochromis niloticus
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Efficacy of live attenuated vaccine derived from the Streptococcus agalactiae on the immune responses of Oreochromis niloticus

机译：生物减毒疫苗衍生自链球菌嗜毒症对牛蒡子的免疫应答的疗效

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Streptococcus agalactiae species have been recognized as the main pathogen causing high mortality in fish leading to significant worldwide economical losses to the aquaculture industries. Vaccine development has become a priority in combating multidrug resistance in bacteria; however, there is a lack of commercial live attenuated vaccine (LAV) against S. agalactiae in Malaysia. The aim of this study is to compare two methods using attenuated bacteria as live vaccine and to evaluate the efficacy of selected LAV on the immune responses and resistance of Oreochromis niloticus (tilapia) against S. agalactiae. The LAV derived from S. agalactiae had been weakened using the chemical agent Acriflavine dye (LAV1), whereas the second vaccine was weakened using serial passages of bacteria on broth media (LAV2). Initial immunization was carried out only on day one, given twice-in the morning and evening, for the 42 day period. Serum samples were collected to determine the systemic antibody (IgM) responses and lysozymal (LSZ) activity using ELISA. On day 43 after immunization, the fish were injected intraperitoneally (i.p) with 0.1 mL of S. agalactiae at LD50 = 1.5 x 10(5) (CFU)/fish. Fish were monitored daily for 10 days. Clinical signs, mortality and the relative percent of survival (RPS) were recorded. Trial 1 results showed a significant increased (P 0.05) in serum IgM titers and LSZ activity as compared to LAV2 and the control group (unvaccinated fish). The efficacy of LAV1 was proven effective as determined by the RPS values, LAV1 at 81.58% as compared to LAV2 at 65.79%. Trial 2 of LAV1 and control group were further determined by administering primary and booster doses revealed a RPS value for LAV1 of 82.05%, with the significant enhancement on the immune responses of tilapia as compared to control group. In conclusion, LAV revealed to elevate antibody IgM levels, LSZ activity and provide long-term protection when added to feed. LAV is a low-cost vaccine shown to rapidly increase the immune response of fish and increase survival rates of fish against S. agalactiae infection.
机译：链球菌嗜醛物种被认为是导致鱼类高死亡率的主要病原体，导致水产养殖产业的全球经济损失。疫苗开发已成为对抗细菌的多药抗性的优先事项;然而，在马来西亚缺乏商业活病变疫苗（LAV）。本研究的目的是将两种方法与活性疫苗相比，作为活疫苗，并评估选定的防晒剂对嗜酸性嗜酸性嗜酸性嗜酸性嗜酸性的免疫应答和抗性的疗效。衍生自S.AlAlactiae的熔融剂使用化学试剂Acriflavine染料（Lav1）削弱，而使用肉汤培养基（Lav2）的细菌串行通道减弱第二疫苗。初步免疫仅在第一个，早上两次和晚上给予的第42天。收集血清样品以确定使用ELISA的全身抗体（IgM）应答和溶菌酶（LSZ）活性。在免疫后的第43天，用0.1mL的LD50 = 1.5×10（5）（CFU）/鱼的0.1ml S.AcAlactiae腹膜内（I.P）注射鱼。每天监测鱼10天。记录了临床症状，死亡率和存活率的相对百分比（RPS）。试验1结果表明，与Lav2和对照组（未接种的鱼类）相比，血清IgM滴度和LSZ活性的显着增加（P <0.05）。通过RPS值确定，Lav1的功效是有效的，与RAV1为81.58％，与Lav2相比为65.79％。通过施用初级和增强剂进一步确定Lav1和对照组的试验2，揭示Lav1的RPS值为82.05％，与对照组相比，罗非鱼的免疫应答显着提高。总之，熔融液显示为升高抗体IgM水平，LSZ活性，并在添加以饲料时提供长期保护。 LAV是一种低成本的疫苗，表明迅速增加鱼的免疫应答，并增加鱼类对嗜毒乳糖感染的鱼类生存率。

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