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首页> 外文期刊>Microbial Pathogenesis >Antimicrobial resistance of three common molecularly identified pathogenic bacteria to Allium aqueous extracts
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Antimicrobial resistance of three common molecularly identified pathogenic bacteria to Allium aqueous extracts

机译：三种常见分子鉴定的肝癌含有含水萃取物的抗菌抗性

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摘要

The aim of this work was to evaluate the in vitro bacterial inhibition of different types of garlic on Escherichia coli ATCC 25922, Listeria monocytogenes and Staphylococcus aureus. The bacterial strains were molecularly identified using gen 16S rDNA molecular identification. Four different types of garlics were used: 1) white, 2) Japanese, 3) elephant and 3) black, and these were evaluated at two different concentrations (0.25 and 0.125 g/mL) per garlic type. Bioactive compounds present in the garlics were identified using high-performance liquid chromatography coupled to ultraviolet detector (HPLC-UV), and total polyphenols were quantified by the Folin-Ciocalteu technique. The Kirby-Bauber method was used for the bacterial evaluation. Aqueous extract of black garlic had the highest amount of polyphenols 6.26 +/- 0.21 mg GAE/mL. The area of inhibition was measured and classified as sensitive, intermediate or resistant. Using the disc diffusion assay, higher concentration (0.25 g/mL) of aqueous extract of white garlic had the highest antibacterial activity area, with 21.46 +/- 3.94 mm for L. monocytogenes, 20.61 +/- 2.47 mm for S. aureus and 17.83 +/- 2.21 mm for E. coli. White garlic had comparable antimicrobial activity as the control (tetracycline at 30 mu g) as indicated by the size of the inhibition halos. Based on your results, white garlic can be used as an alternative to synthetic antimicrobials.
机译：这项工作的目的是评估对大肠杆菌ATCC 25922，李斯特菌单核细胞增生和金黄色葡萄球菌李斯特里西亚李斯特氏菌不同类型大蒜的体外细菌抑制。使用Gen 16S RDNA分子鉴定分子鉴定细菌菌株。使用四种不同类型的大蒜：1）白色，2）日语，3）大象和3）黑色，每蒜类（0.25和0.125g / ml）评价它们。使用高性能液相色谱法鉴定到大蒜中存在的生物活性化合物，偶联至紫外检测器（HPLC-UV），通过Folin-Ciocalteu技术量化总多酚。 Kirby-Bauber方法用于细菌评估。黑色大蒜水提取物具有最多的多酚6.26 +/- 0.21mg / ml。测量抑制面积并分类为敏感，中间体或抗性。使用盘扩散测定，较高浓度（0.25g / ml）的白色大蒜水提取物具有最高的抗菌活性面积，L. monocytogenes的21.46 +/- 3.94mm，对于金黄色葡萄球菌20.61 +/- 2.47mm。大肠杆菌17.83 +/- 2.21 mm。白蒜具有与抑制晕晕的尺寸所示的对照（四环素为30μg）的对照（四环素）。根据您的结果，白蒜可用作合成抗微生物的替代品。

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