Preparation, crystallization, and preliminary X-ray diffraction study of mutant carboxypeptidase T containing the primary specificity pocket of carboxypeptidase B

Akparov V.Kh.; Grishin A.M.; Timofeev V.I.; Kuranova I.P.

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Preparation, crystallization, and preliminary X-ray diffraction study of mutant carboxypeptidase T containing the primary specificity pocket of carboxypeptidase B

机译：含有羧肽酶B一级特异性口袋的突变型羧肽酶T的制备，结晶和初步X射线衍射研究

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Recombinant G215S, A251G, T257A, D260G, T262D mutant carboxypeptidase T from Thermoactinomyces vulgaris containing mutations in the primary specificity pocket was prepared and crystallized. Single crystals with a size of up to 0.3 mm were grown and investigated by X-ray diffraction. Recombinant mutant carboxypeptidase T containing the primary specificity subsite compositionally identical to that of pancreatic carboxypeptidase B crystallizes in the same space group as the natural enzyme. The crystals belong to sp. gr. P6 _322; the unit-cell parameters are a = b = 157.867 ?, c = 104.304 ?, α = β = 90°, γ = 120°. X-ray diffraction data suitable for determining the three-dimensional structure at atomic resolution were collected from one crystal.

机译：制备了来自寻常嗜热放线菌的重组G215S，A251G，T257A，D260G，T262D突变羧肽酶T，该重组羧肽酶T的一级特异性口袋中含有突变并结晶。生长尺寸最大为0.3mm的单晶并通过X射线衍射研究。包含与胰腺羧肽酶B组成相同的主要特异性亚位点的重组突变体羧肽酶T，在与天然酶相同的空间群中结晶。晶体属于sp。 gr。 P6 _322;晶胞参数为：a ＝ b ＝157.867Ω，c ＝104.304Ω，α＝β＝ 90°，γ＝ 120°。从一个晶体中收集适合于以原子分辨率确定三维结构的X射线衍射数据。

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《Crystallography reports》 |2010年第5期|共4页
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Akparov V.Kh.; Grishin A.M.; Timofeev V.I.; Kuranova I.P.;
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中图分类晶体学;
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1. Preparation, crystallization, and preliminary X-ray diffraction study of mutant carboxypeptidase T containing the primary specificity pocket of carboxypeptidase B [J] . Akparov V.Kh., Grishin A.M., Timofeev V.I., Crystallography reports . 2010,第5期

机译：含有羧肽酶B一级特异性口袋的突变型羧肽酶T的制备，结晶和初步X射线衍射研究
2. Crystallization and Preliminary X-ray Diffraction Study of a Mutant of L-Asparaginase from Wolinella succinogenes [J] . Timofeev V. I., Bulushova N. V., Zhukhlistova N. E., Crystallography reports . 2019,第6期

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3. Crystallization and preliminary X-ray diffraction studies of Tyr167His mutant alpha-cyclodextrin glucanotransferase from Bacillus macerans [J] . Yue Yang, Liu Shengquan, Li Hongbin, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第10期

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6. Preparation crystallization and preliminary X-ray diffraction studies of the glycosylated form of human interleukin-23 [O] . Takumi Shirouzono, Mami Chirifu, Chiharu Nakamura, 2012

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Preparation, crystallization, and preliminary X-ray diffraction study of mutant carboxypeptidase T containing the primary specificity pocket of carboxypeptidase B

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