Myelinating cocultures of rodent stem cell line‐derived neurons and immortalized Schwann cells

Ishii Tomohiro; Kawakami Emiko; Endo Kentaro; Misawa Hidemi; Watabe Kazuhiko

首页> 外文期刊>Neuropathology: official journal of the Japanese Society of Neuropathology >Myelinating cocultures of rodent stem cell line‐derived neurons and immortalized Schwann cells

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Myelinating cocultures of rodent stem cell line‐derived neurons and immortalized Schwann cells

机译：啮齿动物干细胞系衍生神经元和永生化施曼细胞的髓鞘细胞

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摘要

Myelination is one of the most remarkable biological events in the neuron–glia interactions for the development of the mammalian nervous system. To elucidate molecular mechanisms of cell‐to‐cell interactions in myelin synthesis in vitro , establishment of the myelinating system in cocultures of continuous neuronal and glial cell lines are desirable. In the present study, we performed co‐culture experiments using rat neural stem cell‐derived neurons or mouse embryonic stem (ES) cell‐derived motoneurons with immortalized rat IFRS1 Schwann cells to establish myelinating cultures between these cell lines. Differentiated neurons derived from an adult rat neural stem cell line 1464R or motoneurons derived from a mouse ES cell line NCH4.3, were mixed with IFRS1 Schwann cells, plated, and maintained in serum‐free F12 medium with B27 supplement, ascorbic acid, and glial cell line‐derived neurotrophic factor. Myelin formation was demonstrated by electron microscopy at 4?weeks in cocultures of 1464R‐derived neurons or NCH4.3‐derived motoneurons with IFRS1 Schwann cells. These in vitro coculture systems utilizing the rodent stable stem and Schwann cell lines can be useful in studies of peripheral nerve development and regeneration.

机译：Myelinal是神经元 - 胶质增长术语中最显着的生物事件之一，用于哺乳动物神经系统的发展。为了阐明体外髓鞘合成细胞对细胞相互作用的分子机制，需要建立连续神经元和胶质细胞系的共培布中的髓鞘系统。在本研究中，我们使用大鼠神经干细胞衍生的神经元或小鼠胚胎茎（ES）细胞衍生的运动神经元进行共培养实验，具有永生化的大鼠IFRS1 Schwann细胞，以在这些细胞系之间建立髓鞘培养物。与来自小鼠ES细胞系NCH4.3的成年大鼠神经干细胞系1464r或衍生自小鼠ES细胞系NCH4.3的运动神经元的分化神经元与IFRS1 Schwann细胞混合，镀层，并用B27补充剂，抗坏血酸和维持在无血清F12培养基中胶质细胞系衍生的神经营养因子。通过电子显微镜形成髓鞘形成，在1464R衍生的神经元或NCH4.3衍生的运动神经元，具有IFRS1 Schwann细胞的培养基中的4℃。利用啮齿动物稳定茎和施旺细胞系的这些体外共蜂窝系统可用于外周神经发育和再生的研究。

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来源
《Neuropathology: official journal of the Japanese Society of Neuropathology》 |2017年第5期|共7页
作者
Ishii Tomohiro; Kawakami Emiko; Endo Kentaro; Misawa Hidemi; Watabe Kazuhiko;
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作者单位

Laboratory for Neurodegenerative PathologyTokyo Metropolitan Institute of Medical ScienceSetagaya;

Laboratory for Neurodegenerative PathologyTokyo Metropolitan Institute of Medical ScienceSetagaya;

Center for Basic Technology ResearchTokyo Metropolitan Institute of Medical ScienceSetagaya Tokyo;

Department of PharmacologyKeio University Faculty of PharmacyMinato Tokyo Japan;

Laboratory for Neurodegenerative PathologyTokyo Metropolitan Institute of Medical ScienceSetagaya;

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正文语种 eng
中图分类神经病学与精神病学;
关键词
coculture techniques; embryonic stem cells; myelin sheath; neural stem cells; Schwann cells;

机译：共培养技术;胚胎干细胞;髓鞘;神经干细胞;施万细胞;

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专利

1. Myelinating cocultures of rodent stem cell line‐derived neurons and immortalized Schwann cells [J] . Ishii Tomohiro, Kawakami Emiko, Endo Kentaro, Neuropathology: official journal of the Japanese Society of Neuropathology . 2017,第5期

机译：啮齿动物干细胞系衍生神经元和永生化施曼细胞的髓鞘细胞
2. Expression of neurofilament L-promoter green-fluorescent protein constructs in immortalized Schwann cell-neuron coculture. [J] . Haynes LW, Schmitz S, Clegg JC, Neuroscience Letters: An International Multidisciplinary Journal Devoted to the Rapid Publication of Basic Research in the Brain Sciences . 1999,第3期

机译：神经丝L启动子绿色荧光蛋白构建体在永生化的Schwann细胞-神经元共培养物中的表达。
3. Spontaneously immortalized Schwann cells from adult Fischer rat as a valuable tool for exploring neuron-Schwann cell interactions. [J] . Sango K, Yanagisawa H, Kawakami E, Journal of Neuroscience Research . 2011,第6期

机译：成年Fischer大鼠自发永生化的Schwann细胞是探索神经元-Schwann细胞相互作用的宝贵工具。
4. Induction of intrafusal muscle fibers from human satellite cells and their innervations by human stem cell-derived sensory neurons [C] . Xiufang Guo, Alisha Colon, Candace Martin, Society for Biomaterials annual meeting and exposition . 2015

机译：人类卫星细胞衍生的感觉神经元诱导人类卫星细胞融合神经内纤维及其神经支配
5. The neural crest origins of skin-derived precursors: An accessible source of myelinating Schwann cells. [D] . McKenzie, Ian. 2006

机译：皮肤来源的前体的神经：起源：髓鞘雪旺细胞的可及来源。
6. Skin-Derived Precursors Generate Myelinating Schwann Cells for the Injured and Dysmyelinated Nervous System [O] . Ian A. McKenzie, Jeff Biernaskie, Jean G. Toma, 2006

机译：皮肤衍生的前体产生受伤的和髓鞘化的神经系统的髓鞘雪旺细胞。
7. Myelinating cocultures of rodent stem cell line-derived neurons and immortalized Schwann cells [O] . Tomohiro Ishii, Emiko Kawakami, Kentaro Endo, 2017

机译：啮齿动物干细胞系衍生神经元和永生化施曼细胞的髓鞘细胞

Myelinating cocultures of rodent stem cell line‐derived neurons and immortalized Schwann cells

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