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首页> 外文期刊>Neuropathology: official journal of the Japanese Society of Neuropathology >Aquaporin‐4 expression dynamically varies after acute spinal cord injury‐induced disruption of blood spinal cord barrier in rats
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Aquaporin‐4 expression dynamically varies after acute spinal cord injury‐induced disruption of blood spinal cord barrier in rats

机译：Aquaporin-4表达在急性脊髓损伤引起的大鼠血脊髓屏障中断后动态变化

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摘要

The blood–spinal cord barrier (BSCB) changes badly after spinal cord injury (SCI), and it is an important pathophysiological basis of SCI secondary damage. Aquaporin‐4 (AQP4), one of the transmembrane proteins in spinal cord, has been shown to be closely related to the development of the BSCB and edema. We established a SCI model in rats using a free‐falling weight drop device to subsequently investigate AQP4 expression. AQP4 messenger RNA (mRNA) and protein expression and immunoreactivity were detected in spinal cord tissue using reverse transcription‐real‐time quantitative polymerase chain reaction (RT‐qPCR), immunohistochemistry and Western blot analysis. We found the water content and edema of the spinal cord were significantly higher than the control group after SCI, which was related to the growth of BSCB permeability; both reached their peak on the third day after injury. One, 3, 5, 7?days after injury, the immune response and protein expression in the model group increased from 1 to 3?days, with a plateau period from 3 to 5?days and a decline from 5 to 7?days, showing a significant difference compared with the sham group at each time point ( P ??0.05), while the RT‐qPCR results showed a decline of mRNA just after 3?days. In conclusion, after SCI, the water content of the spinal cord and the BSCB permeability increases, together with the excessive expression of AQP4, which reached a peak on the third day. AQP4 expression is closely relative to the permeability of BSCB and the water content of the spinal cord.
机译：血缘脊髓屏障（BSCB）在脊髓损伤（SCI）后严重变化，并且它是SCI二次损害的重要病理生理基础。 Aquaporin-4（AQP4）是脊髓中的一种跨膜蛋白，已被证明与BSCB和水肿的发育密切相关。我们使用自由下降重量滴装置在大鼠中建立了SCI模型，以便随后调查AQP4表达。使用逆转录实时定量聚合酶链反应（RT-QPCR），免疫组化和Western印迹分析，在脊髓组织中检测到AQP4信使RNA（mRNA）和蛋白质表达和免疫反应性。我们发现SCI后脊髓的水含量和水肿显着高于对照组，这与BSCB渗透性的生长有关;两者都在受伤后第三天达到了巅峰。伤后3,5,7-7天，模型组的免疫应答和蛋白质表达从1到3？天增加，高原时间为3至5？天和5到7日下降？天，与每次时间点的假组相比显示有显着差异（p？＆ 0.05），而RT-QPCR结果显示在3日之后mRNA的下降。总之，在SCI之后，脊髓的含水量和BSCB渗透性增加，与AQP4的过度表达一起增加，这在第三天达到了峰值。 AQP4表达与BSCB的渗透性和脊髓的含水量密切相关。

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