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Role of the arbuscular mycorrhizal symbiosis in tolerance response against Armillaria mellea in lavender

机译:丛枝菌根共生在薰衣草中Armillaria Mellea耐受性耐受性的作用

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摘要

Lavender species form the arbuscular mycorrhizal symbiosis and are at the same time highly susceptible to white root rot. In an attempt to evaluate the response of mycorrhizal Lavandula angustifolia L. to Armillaria mellea (Vahl:Fr) P. Kumm in a greenhouse experiment, plants were previously inoculated with an isolate of the arbuscular mycorrhizal fungus Rhizophagus irregularis (former Glomus intraradices BEG 72) and the influence of the pH growing medium on the plant-symbiont-pathogen interaction was tested in gnotobiotic autotrophic growth systems in which mycorrhizal inoculum was obtained from root organ cultures. After ten months growth dual-inoculated lavender plants grown in containers with a pasteurized substrate mixture produced a similar number of spikes than healthy plants and achieved equivalent plant diameter coverage. When the growing medium in the autotrophic systems was supplemented with calcium carbonate, the inoculation of lavender plantlets with R. irregularis at higher pH (7.0 and 8.5) media caused a significant decrease of A. mellea presence in plant roots, as detected by qPCR. Moreover, the observation of internal root mycorrhizal infection showed that the extent of mycorrhizal colonization increased in plant roots grown at higher pH, indicating that tolerance to white root rot in lavender plants inoculated with R. irregularis could be associated to mycorrhizal establishment.
机译:薰衣草物种形成丛枝菌根共生,同时高易受白根腐蚀的影响。试图评估菌根菌菌菌的响应,血清素菌,麦芽菊(VAHL:FR)P. Kumm在温室实验中,以前用枝氨酸菌根菌株植物的植物感染植物(以前的Glomus Intraradices乞讨72)并且在侏略罗氏自养生长系统中测试了pH生长培养基对植物 - 共生 - 病原体相互作用的影响,其中从根器官培养物中获得菌根接种物。十个月后,生长,在包含巴氏灭菌的基质混合物中生长的双接种薰衣草植物产生了与健康植物相似的穗状花序,并获得了等同的植物直径覆盖率。当碳酸钙的生长培养基补充有碳酸钙时,在较高pH(7.0和8.5)培养基中接种具有R.不规则的薰衣草植物在植物根部中的显着降低,如QPCR检测到的。此外,内部根系菌根感染的观察结果表明,在较高的pH下种植的植物根中腐蚀性殖民的程度增加,表明在接种R. Irregularis的薰衣草植物中对白根腐蚀的耐受性可能与菌根建立相关。

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