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首页> 外文期刊>Pharmacology, Biochemistry and Behavior >The light-dark task in zebrafish confuses two distinct factors: Interaction between background shade and illumination level preference
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The light-dark task in zebrafish confuses two distinct factors: Interaction between background shade and illumination level preference

机译：斑马鱼中的浅黑暗任务使两个不同的因素混淆：背景阴影和照明水平偏好之间的相互作用

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The light-dark preference task has been commonly used in rodents to screen for anxiogenic and anxiolytic drugs. However, recent adaptations of the light-dark preference test for zebrafish have produced inconsistent results. Several studies have reported that zebrafish exhibit a preference for light, while others have found a preference for black. We suggest the inconsistencies may be the result of confusing certain parameters of the test leading to improper interpretation. For example, researchers often use "light" interchangeably with "white" and "dark" with "black" when these are two distinct factors: level of illumination vs. background shade. In the current study, we use specifically designed preference tanks to investigate the influence of background shade (i.e. white vs. black) and level of illumination (i.e. light vs. dark) on preference and anxiety-like behaviour. Furthermore, we pharmacologically validate our results by quantifying the effects of ethanol, a drug with known anxiety-altering properties, on anxiety-like behaviours. Here we report that zebrafish's preference varies depending upon background shade and level of illumination. We also found that ethanol administration altered behavioural responses in an illumination- and background shade-dependent manner. Our findings reinforce the need to correctly differentiate between these factors when interpreting results obtained with this behavioural paradigm. Lastly, our results show that simple modifications to the experimental tank in which anxiety-related responses are measured can significantly alter behaviour of zebrafish, supporting the need for standardized testing procedures and/or for detailed description of experimental procedures and the apparatus.
机译：光暗偏好任务通常用于啮齿动物以筛选焦炭和抗焦油药。然而，最近对斑马鱼的光暗偏好测试的适应性产生了不一致的结果。几项研究报道，斑马鱼表现出光明的偏好，而其他研究则发现了黑色的偏好。我们建议不一致可能是令人困惑某些测试参数的结果，从而导致解释不当。例如，研究人员通常使用“白色”和“黑暗”，当这些是两个不同的因素时，“黑色”和“黑暗”：照明水平与背景阴影。在目前的研究中，我们使用专门设计的偏好坦克来调查背景阴影（即白色与黑色）和照明水平（即光与暗）对偏好和焦虑的行为的影响。此外，我们通过量化乙醇，一种具有已知焦虑的特性的药物的影响，药理学验证我们的结果。在这里，我们报告说，斑马鱼的偏好因背景阴影和照明水平而异。我们还发现乙醇给药在照明和背景阴影依赖方式中改变了行为响应。我们的研究结果加强了在用这种行为范式获得的结果解释结果时正确地区分这些因素。最后，我们的结果表明，对测量焦虑相关响应的实验罐的简单修改可以显着改变斑马鱼的行为，支持对实验程序和装置的详细描述的需要和/或详细描述。

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