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首页> 外文期刊>BJU international >The role of the urothelium in mediating bladder responses to isoprenaline.
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The role of the urothelium in mediating bladder responses to isoprenaline.

机译：尿路上皮在介导膀胱对异丙肾上腺素反应中的作用。

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OBJECTIVES: To investigate whether the responses of the pig bladder to isoprenaline (a nonselective beta-adrenoceptor agonist) are influenced by the presence of an intact urothelium and whether any influence might be attributed to the release of nitric oxide (NO), since stimulation of beta-adrenoceptors induces a direct relaxation of detrusor smooth muscle and beta-adrenoceptors are also present on the urothelium. MATERIAL AND METHODS: Paired (in the presence or absence of urothelium) longitudinal strips of pig bladder dome were set up in tissue baths and the developed tension recorded. Relaxation responses to isoprenaline were examined after pre-contraction with carbachol. The inhibitory effects of isoprenaline were examined by comparing responses to carbachol in the absence and presence of isoprenaline. To examine a possible role for NO, similar experiments were performed in the presence of the NO synthase inhibitor N(G)-nitro-L-arginine (L-NNA). RESULTS: In the presence of the urothelium, both the potency (pEC(50)) and the maximum contractile responses to carbachol were depressed. In relaxation experiments, isoprenaline relaxed carbachol pre-contracted tissues by approximately 75%, and the potency and maximum relaxation were similar in the absence and presence of the urothelium. In the inhibition experiments, the presence of isoprenaline caused rightward parallel shifts of the concentration-response curves to carbachol, but isoprenaline did not influence the maximum contractions. In the presence of the urothelium there was a greater shift with 0.1 microm isoprenaline than in denuded tissues. Incubation with L-NNA did not affect the influence of the urothelium on responses to isoprenaline in any experimental group. CONCLUSIONS: The relaxation responses of the bladder to isoprenaline do not appear to involve the urothelium or NO release in vitro. However, contractile responses to carbachol were inhibited in the presence of an intact urothelium, and this might reflect the release of an inhibitory factor other than NO.
机译：目的：研究完整尿路上皮的存在是否影响猪膀胱对异丙肾上腺素（一种非选择性的β-肾上腺素受体激动剂）的反应，以及是否有任何影响可能归因于一氧化氮的刺激释放一氧化氮（NO） β-肾上腺素能诱导逼尿肌平滑肌直接松弛，β-肾上腺素能受体也存在于尿路上皮。材料与方法：在组织浴中设置成对的（在有或没有尿道上皮的情况下）猪膀胱穹顶的纵向条，并记录所产生的张力。用卡巴胆碱预收缩后检查对异丙肾上腺素的松弛反应。通过比较在不存在和存在异丙肾上腺素时对卡巴胆碱的反应来检查异丙肾上腺素的抑制作用。为了检查NO的可能作用，在NO合酶抑制剂N（G）-硝基-L-精氨酸（L-NNA）存在下进行了类似的实验。结果：在存在尿路上皮时，对卡巴胆碱的效力（pEC（50））和最大收缩反应均被抑制。在舒张实验中，异丙肾上腺素使卡巴胆碱预收缩的组织舒张了大约75％，在不存在和存在尿路上皮的情况下，效价和最大舒张相似。在抑制实验中，异丙肾上腺素的存在导致浓度-响应曲线向右向右平移，向卡巴胆碱转移，但异丙肾上腺素不影响最大收缩。在有尿路上皮的情况下，0.1μm异丙肾上腺素比裸露组织有更大的位移。在任何实验组中，与L-NNA一起孵育均不会影响尿路上皮对异丙肾上腺素反应的影响。结论：膀胱对异丙肾上腺素的松弛反应似乎不涉及尿路上皮或NO的释放。但是，在完整的尿路上皮中，对胆碱的收缩反应受到抑制，这可能反映了除NO以外的抑制因子的释放。

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