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首页> 外文期刊>The European Journal of Neuroscience >The Ventral Tegmental Area has calbindin neurons with the capability to co-release glutamate and dopamine into the nucleus accumbens
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The Ventral Tegmental Area has calbindin neurons with the capability to co-release glutamate and dopamine into the nucleus accumbens

机译：腹侧腹部面积具有Calbindin神经元，具有将谷氨酸和多巴胺共同释放到核心腺中的能力

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The ventral tegmental area (VTA) has three major classes of neurons: dopaminergic (expressing tyrosine hydroxylase; TH), GABAergic (expressing vesicular GABA transporter; VGaT) and glutamatergic (expressing vesicular glutamate transporter 2; VGluT2). While VTA dopaminergic and GABAergic neurons have been further characterized by expression of calcium-binding proteins (calbindin, CB; calretinin, CR or parvalbumin, PV), it is unclear whether these proteins are expressed in rat VTA glutamatergic neurons. Here, by a combination of in situ hybridization (for VGluT2 mRNA detection) and immunohistochemistry (for CB-, CR- or PV-detection), we found that among the total population of VGluT2 neurons, 30% coexpressed CB, 3% coexpressed PV and <1% coexpressed CR. Given that some VGluT2 neurons coexpress TH or VGaT, we examined whether these neurons coexpress CB, and found that about 20% of VGluT2-CB neurons coexpressed TH and about 13% coexpressed VGaT. Because VTA TH-CB neurons are known to target the nucleus accumbens (nAcc), we determined whether VGluT2-CB-TH neurons innervate nAcc, and found that about 80% of VGluT2-CB neurons innervating the nAcc shell coexpressed TH. In summary, (a) CB, PV and CR are detected in subpopulations of VTA-VGluT2 neurons; (b) CB is the main calcium-binding protein present in VTA-VGluT2 neurons; (c) one-third of VTA-VGluT2 neurons coexpress CB; (d) some VTA-VGluT2-CB neurons have the capability to co-release dopamine or GABA, and (e) a subpopulation of VTA glutamatergic-dopaminergic neurons innervates nAcc shell. These findings further provide evidence for molecular diversity among VTA-VGluT2 neurons, neurons that may play a role in specific circuitry and behaviours.
机译：腹侧面积（VTA）具有三个主要的神经元类：多巴胺能（表达酪氨酸羟化酶; TH），加布枸杞（表达囊泡GABA转运蛋白; VGAT）和谷氨酸盐酰胺（表达凹凸谷氨酸转运蛋白2; VGLUT2）。虽然VTA多巴胺能和加法比的神经元进一步通过表达钙结合蛋白（Calbindin，Cb; Calretinin，Cr或Parvalbumin，PV），但目前尚不清楚这些蛋白质是否在大鼠VTA谷氨酸谷氨酸神经元中表达。这里，通过原位杂交（用于VGLut2 mRNA检测）和免疫组织化学（用于CB-，Cr-或PV检测），我们发现在VGLut2神经元的总群中，30％的共同抑制CB，3％共存PV和<1％的共用CR。鉴于某些VGLut2神经元共表达TH或VGAT，我们检查了这些神经元是否共用CB，发现大约20％的VGLut2-CB神经元共表达TH和约13％的共同表达VGAT。因为已知VTA TH-CB神经元靶向核毒核（NACC），所以我们确定了VGLut2-CB-TH神经元的关联NACC，发现约80％的VGLut2-CB神经元检查NACC壳组成的NACC壳。发明内容中，在VTA-VGLut2神经元的亚群中检测到（a）Cb，PV和Cr; （b）Cb是VTA-Vglut2神经元中存在的主要钙结合蛋白; （c）vta-Vglut2神经元的三分之一CB; （d）一些VTA-Vglut2-CB神经元具有共同释放多巴胺或GABA的能力，并且（e）VTA谷氨酰胺 - 多巴胺能神经元的亚级分配NACC壳。这些发现还提供了VTA-VGLut2神经元，神经元在特定电路和行为中发挥作用的神经元的分子多样性的证据。

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