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首页> 外文期刊>AIDS Research and Human Retroviruses >Human Immunodeficiency Virus Coinfection with Hepatitis B Virus Leads to a Decrease in Extracellular and Intracellular Hepatitis B Antigen
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Human Immunodeficiency Virus Coinfection with Hepatitis B Virus Leads to a Decrease in Extracellular and Intracellular Hepatitis B Antigen

机译：人免疫缺陷病毒与乙型肝炎病毒的共同感染导致细胞外和细胞内乙型肝炎抗原的减少

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摘要

Chronic hepatitis B virus (HBV) infection could cause severe liver disease including cirrhosis, hepatocellular carcinoma, and end-stage liver failure in HIV-positive individuals. The available data from clinical studies suggest that HIV infection modulates the HBV-specific T cell response. However, the virological and molecular aspects of HIV-HBV coinfection are currently poorly understood due to the lack of appropriate model systems. In this study, the effect of HIV infection on the life cycle of HBV was explored using an in vitro model system. The present data show that the extracellular and intracellular hepatitis B surface antigen (HBsAg) and e antigen (HBeAg) decrease significantly in HepG2 cells cotransfected with HIV NL4-3 and pHBV1.3 as compared to those cells transfected only with pHBV1.3. Moreover, a significant decrease in HBV DNA and mRNA expression was also observed in the cotransfected cells. HIV Rev protein, an RNA-bound regulatory protein, could significantly decrease the expression levels of extracellular and intracellular HBsAg and HBeAg by mediating the expression of HBV mRNA in cells cotransfected with plasmids containing HIV-1 Rev and pHBV1.3. Further experiments demonstrate that HIV Rev manipulated neither the promoters of HBV nor the nuclear export of HBV mRNA. These results from the in vitro model system might provide clues to further understand the rapid progression of liver disease in HIV-HBV-coinfected patients.
机译：慢性乙型肝炎病毒（HBV）感染可能导致严重的肝脏疾病，包括肝硬化，肝细胞癌和HIV阳性个体的终末期肝衰竭。临床研究的可用数据表明，HIV感染可调节HBV特异性T细胞反应。但是，由于缺乏适当的模型系统，目前对HIV-HBV合并感染的病毒学和分子学方面了解甚少。在这项研究中，使用体外模型系统探索了HIV感染对HBV生命周期的影响。本数据表明，与仅用pHBV1.3转染的细胞相比，在HIV NL4-3和pHBV1.3共转染的HepG2细胞中，细胞外和细胞内的乙型肝炎表面抗原（HBsAg）和e抗原（HBeAg）显着降低。而且，在共转染的细胞中也观察到了HBV DNA和mRNA表达的显着降低。 HIV Rev蛋白是一种与RNA结合的调节蛋白，它可以通过介导与HIV-1 Rev和pHBV1.3质粒共转染的细胞中HBV mRNA的表达来显着降低细胞外和细胞内HBsAg和HBeAg的表达水平。进一步的实验表明，HIV Rev既不操纵HBV的启动子，也不操纵HBV mRNA的核输出。体外模型系统的这些结果可能为进一步了解HIV-HBV合并感染的患者肝脏疾病的快速发展提供线索。

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