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首页> 外文期刊>Biotechnology Progress >A Feasible Enzymatic Process for D-Tagatose Production by an Immobilized Thermostable L-Arabinose Isomerase in a Packed-Bed Bioreactor
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A Feasible Enzymatic Process for D-Tagatose Production by an Immobilized Thermostable L-Arabinose Isomerase in a Packed-Bed Bioreactor

机译：固定床型生物反应器中固定的热稳定L-阿拉伯糖异构酶生产D-塔格糖的可行酶法

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摘要

To develop a feasible enzymatic process for D-tagatose production,a thermostable L-arabinose isomerase,Gali152 was immobilized in alginate,and the galactose isomerization reaction conditions ere optimized.The pH and temperature for the mzximal galactose isomerization reactioj were pH 8.0 and 65 deg C in the immobilized enzyme system and pH 7.5 and 60 deg C in the free enzyme system.The presence of manganese ion enhanced glactose isomeriation of tagatise in both the free and immobilized enzyme systems.The immobilized enzyme was more stable than the free enzyme at the same pH and temperature.Under stable conditions of pH 8.0 and 60 deg C the immobilized enzyme produced 58 g/L of tagatose from 100 g/L galactose in 90 h by batch reaction,whereas the free enzyme produced 37 g/L tagatose due to its lower stability.Apacked-bed bioreactor with immobilized Gali152 in alginate beads produced 50g/L tagatose from 100 g/L galactose in 168 h,with a productivity fo 13.3 (g of tagatose(/(L-reactor.h) in continuous mode.The bioreactor produced 230 g/L tagatose from 500 g/L galactose in continuous recycline mode,with a productivity of 9.6g/(L.h) and a conversion yield of 46%.
机译：为了开发可行的酶法生产D-塔格糖，将热稳定的L-阿拉伯糖异构酶固定在海藻酸盐中，对Gali152进行固定，优化半乳糖异构化反应的条件。最短的半乳糖异构化反应的pH和温度为pH 8.0和65℃固定化酶体系中的C以及游离酶体系中的pH 7.5和60摄氏度。锰离子的存在增强了游离酶和固定化酶体系中tagatise的乳糖异构化。固定化酶比游离酶更稳定在pH值8.0和60摄氏度的稳定条件下，固定化酶在90小时内通过间歇反应从100克/升半乳糖产生58克/升的塔格糖，而游离酶则通过间歇反应产生了37克/升的塔格糖。将固定化Gali152固定在藻酸盐珠粒中的床式生物反应器在168小时内由100 g / L半乳糖产生50g / L塔格糖，生产力为13.3（g塔格糖（/(L-reactor.h）该生物反应器在连续循环模式下从500 g / L半乳糖中产生230 g / L塔格糖，生产率为9.6g /（L.h），转化率为46％。

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