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首页> 外文期刊>Biotechnology Progress >Different properties of the lipases contained in porcine pancreatic lipase extracts as enantioselective biocatalysts
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Different properties of the lipases contained in porcine pancreatic lipase extracts as enantioselective biocatalysts

机译：猪胰脂肪酶提取物中所含脂肪酶作为对映选择性生物催化剂的不同性质

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摘要

The porcine pancreatic lipase (PPL) extracts contain a mixture of several lipases. Their fractioning was performed by sequential adsorption via interfacial activation on supports with different hydrophobicity. A protein of 25 KDa was preferentially adsorbed on octyl-Sepharose, another protein of 33 kDa was mainly adsorbed on octadecyl-Sepabeads support, and the PPL was mainly adsorbed on the support bearing phenyl groups. The different immobilized preparations showed different properties and different response due to change in the experimental conditions. Thus, in the hydrolysis of (+/-)-2-hydroxy-4-phenylbutyric acid ethyl ester [(+/-)-1] to produce the corresponding acid [2], the octyl-25KDa preparation showed the best enantioselectivity (E) value (E = 7) at pH 5 and 25 degreesC, whereas the phenyl-PPL was the most enantioselective (E = 10) at pH 5, 4 degreesC, and 10% dioxane. Using different preparations at different pHs it was possible to resolve (+/-)-2-O-butyryl-2-phenylacetic acid [(+/-)-3] with a high E value (E > 100); for example, with octadecyl-33 KDa enzyme at pH 8.
机译：猪胰脂肪酶（PPL）提取物含有几种脂肪酶的混合物。它们的分馏是通过在不同疏水性的载体上通过界面活化依次吸附来进行的。 25 KDa的蛋白质优先吸附在辛基-琼脂糖上，另一种33 kDa的蛋白质主要吸附在十八烷基-Sepabeads载体上，而PPL主要吸附在带有苯基的载体上。由于实验条件的变化，不同的固定制剂显示出不同的性质和不同的响应。因此，在水解（+/-）-2-羟基-4-苯基丁酸乙酯[（+/-）-1]以产生相应的酸[2]时，辛基25KDa制剂显示出最佳的对映选择性（ E）在pH 5和25摄氏度下的值（E = 7），而苯基PPL在pH 5、4摄氏度和10％的二恶烷条件下对映选择性最高（E = 10）。使用不同pH值的不同制剂，可以拆分出具有较高E值（E> 100）的（+/-）-2-O-丁酰基-2-苯基乙酸[（+/-）-3]。例如，使用pH 8的十八烷基33 KDa酶。

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