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首页> 外文期刊>Biotechnology Progress >Analysis of the Phase Behavior of the Aqueous Poly(ethylene glycol)-Ficoll System
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Analysis of the Phase Behavior of the Aqueous Poly(ethylene glycol)-Ficoll System

机译：聚乙二醇-Ficoll水溶液体系的相行为分析

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The PEG-Ficoll polymer phase system is one that has been overlooked in the past for biotechnology applications because of the stability of its emulsions. However, new applications, such as emulsion coating of cells, are appearinfg that rely on this very property. Ficoll is highly polydisperse and multimodal wiht three distinct Ficoll peaks in gel permeation chromatography. As a result, the transition between one-phase and two-phase systems is blurred and the binodials obtained through turbidometric titration and tie-line analysis differ significantly. Moreover, since the three Ficoll peaks partition differentily, tie-line analysis cannot be described by a simple model of the aqueous two-phase system. A simple modficiation to the model allowed for excellent fit, and this modification may prove well-suited for the many practical cases where aqueous two-phase systems fail to display parallel tie-lines as implicitly assumed in the simpler model.
机译：PEG-Ficoll聚合物相体系由于其乳液的稳定性而在过去的生物技术应用中被忽略。然而，依赖于这种特性的新应用出现了，例如细胞的乳胶涂层。 Ficoll在凝胶渗透色谱法中具有三个不同的Ficoll峰，具有高度多分散性和多峰性。结果，一相和两相系统之间的过渡变得模糊，并且通过浊度滴定法和联系线分析获得的二项式曲线显着不同。此外，由于三个Ficoll峰的分区不同，因此无法通过水相两相系统的简单模型来描述联系线分析。对模型的简单修改可以实现出色的拟合，并且这种修改可能非常适合水性两相系统无法显示更简单模型中隐含假设的平行联络线的许多实际情况。

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