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首页> 外文期刊>Journal of applied toxicology >Estrogenic effects of fluorinated diiodine alkanes in MCF-7 cells, H295R cells and zebrafish embryo assays
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Estrogenic effects of fluorinated diiodine alkanes in MCF-7 cells, H295R cells and zebrafish embryo assays

机译：氟化二碘烷烃在MCF-7细胞，H295R细胞和斑马鱼胚胎测定中的雌激素作用

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ABSTRACT: Fluorinated diiodine alkanes (FDIAs), important industrial intermediates in the synthesis of various perfluorinated compounds, which are distributed widely in wildlife and humans. Recent studies showed that FDIAs had in vitro estrogenic effects. However, to date, little information is available regarding the in vivo estrogenic effects of FDIAs and the mechanisms are unclear. In this study, a combination of in vitro and in vivo assays was used to investigate the estrogenic effects of FDIAs. We tested the in vitro estrogenic effects and estrogen receptor-related gene expression via MCF-7 cell assay. The hormone level of estradiol and the expression of estrogenic synthesis genes were measured in the H295R cell assay. Finally, the in vivo effects of FDIAs on development and estrogen-related gene expression were assessed in the zebrafish embryos assay. The results demonstrated that FDIAs could exhibit estrogenic activity through inducing cell proliferation (1.6-6.7-fold of the control) and estrogen receptor alpha gene expression (1.07-1.39-fold of the control), altering estradiol production (1.14-1.22-fold of the control) and the major estrogenic synthesis gene expression of CYP19 (1.22-1.31-fold of the control), disrupting the estrogen-related genes (esr1 and cyp19b) levels in zebrafish (1.52-2.99-fold and 2.95-5.00-fold of the control for esr1 and cyp19b, respectively). The current findings indicated the potential estrogenic effects of FDIAs and provided novel information for human risk assessment. ? 2019 John Wiley & Sons, Ltd.
机译：摘要：氟化二碘烷烃（FDIAS），重要的产业中间体在合成各种全氟化化合物中，其在野生动物和人类中广泛分布。最近的研究表明FDIAS具有体外雌激素作用。然而，迄今为止，关于FDIAS的体内雌激素作用的信息很少，并且该机制尚不清楚。在本研究中，使用体外和体内测定的组合来研究FDIAS的雌激素作用。我们通过MCF-7细胞测定测试了体外雌激素效应和雌激素受体相关基因表达。在H295R细胞测定中测量雌二醇的激素水平和雌激素合成基因的表达。最后，在斑马鱼胚胎测定中评估了FDIAS对发育和雌激素相关基因表达的体内效应。结果表明，FDIAS可以通过诱导细胞增殖（1.6-6.7倍）和雌激素受体α基因表达（对照的1.07-1.39倍），改变雌二醇产生（1.14-1.22倍）来表现出雌激素活性（1.14-1.22倍）对照）和CYP19的主要雌激素合成基因表达（对照1.22-1.31倍），破坏斑马鱼（1.52-2.99倍和2.95-5.00倍）中的雌激素相关基因（ESR1和CYP19B）水平分别对ESR1和CYP19B的控制。目前的发现表明FDIAS的潜在雌激素作用，并为人类风险评估提供了新颖的信息。还2019 John Wiley＆Sons，Ltd。

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